Anti-FANCM Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8IYD8

Core Product Specifications and Parameters

Biological Background: FANCM Function and Localization

• FANCM is a DNA-dependent ATPase and integral component of the Fanconi anemia core complex, essential for activating the FA pathway and promoting FANCI-FANCD2 monoubiquitination upon DNA damage. Related references: PMID:16116422 PMID:19423727 PMID:20347428
• In complex with CENPS/CENPX, FANCM binds dsDNA, fork-structured DNA, and Holliday junctions, and its ATP-dependent branch migration activity remodels replication forks; with FAAP24, it preferentially binds ssDNA and splayed-arm substrates. Related references: PMID:20347428 PMID:20347429 PMID:17289582 PMID:16116434
• Localized in the nucleus, consistent with its role in DNA repair and genome maintenance.
• Expressed in germ cells of fetal and adult ovaries, including oogonia and pachytene oocytes, and in the testis. Related references: PMID:29231814 PMID:30075111
• Belongs to the helicase superfamily, with conserved ATP-binding and DNA-binding domains; classified as a phosphoprotein involved in DNA damage response and disease variants linked to Fanconi anemia and premature ovarian failure.
• Multiple isoforms exist due to alternative splicing.

Experimental Guidance and Technical Tips

• For Western blot, use whole cell lysates from human cells and consider extended transfer times due to the large 232 kDa protein size.
• In IF/ICC, verify nuclear staining pattern; fixation and permeabilization conditions should be optimized for nuclear antigens.
• ELISA applications may utilize the recombinant immunogen as a positive control; antibody titer and specificity should be validated in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for DNA repair and Fanconi anemia pathway research. Our one-stop procurement model ensures diagnostic manufacturers and laboratories gain efficient access to high-quality reagents and technical support—from target selection to commercial assay development. Contact us to explore custom sourcing or bulk supply options for your FANCM-related projects.