Anti-FANCI Rabbit Polyclonal Antibody for WB, ELISA - Q9NVI1

Core Product Specifications and Parameters

Biological Background: FANCI Function and Localization

• FANCI (KIAA1794) is essential for DNA repair, promoting FANCD2 monoubiquitination by FANCL and facilitating recruitment to DNA damage sites for interstrand crosslink (ICL) repair and homologous recombination.
  Related references: PMID:17412408, PMID:17460694, PMID:17452773
• The FANCI-FANCD2 complex binds and scans double-stranded DNA, stalling at DNA junctions to detect damage and initiate repair.
  Related references: PMID:19589784
• It participates in S phase and G2 phase checkpoint activation following DNA damage, ensuring cell cycle arrest for repair.
  Related references: PMID:25862789
• FANCI contains DNA-binding regions and undergoes key post-translational modifications, including monoubiquitination and phosphorylation, that regulate its function.
• Multiple isoforms are produced by alternative splicing, potentially impacting protein interactions and stability.
• The protein localizes to both the nucleus and cytoplasm.
• Mutations in the FANCI gene are linked to Fanconi anemia, a disorder hallmarked by bone marrow failure and cancer predisposition.

Experimental Guidance and Technical Tips

• For Western blot, FANCI migrates at approximately 149 kDa; use a positive control such as HeLa or HEK293 total cell lysate. Optimize transfer conditions for high-molecular-weight proteins.
• This polyclonal antibody may recognize multiple isoforms; validate in your specific sample system and consider using a loading control for accurate quantification.
• When performing ELISA, titrate the antibody to determine the optimal working concentration with recombinant FANCI as a standard.
• Pre-absorption with the immunogen can be used to confirm specificity in immunodetection assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized IVD raw-material supplier, offering one-stop access to antibodies, antigens, and ancillary reagents for diagnostic manufacturing and research. Our portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk raw materials to support DNA repair and oncology studies. Leverage our integrated supply chain to efficiently source FANCI antibodies and rare-target reagents, accelerating your project from concept to clinical application.