Anti-FANCG Rabbit Polyclonal Antibody for WB, ELISA - O15287

Core Product Specifications and Parameters

Biological Background: FANCG Function and Localization

• FANCG (Fanconi anemia group G protein), also designated DNA repair protein XRCC9, is a key component of the Fanconi anemia (FA) pathway involved in the maintenance of genomic stability.
• It functions in postreplication DNA repair and cell cycle checkpoint control, particularly in the repair of interstrand DNA cross-links, and is considered a candidate tumor suppressor.
• The protein is encoded by the FANCG gene (also known as XRCC9) on chromosome 9, and mutations in this gene are associated with Fanconi anemia, a genetic disorder characterized by bone marrow failure and cancer predisposition.
• Subcellularly, FANCG localizes to both the nucleus and the cytoplasm, facilitating its dynamic role in DNA damage response and repair complex assembly.
• It is highly expressed in testis and thymus, with detectable levels in lymphoblastoid cells, suggesting a tissue-specific demand for DNA cross-link repair in highly proliferative or meiotic tissues.
• Structural features include tetratricopeptide repeat (TPR) motifs, which mediate protein-protein interactions, and the protein is regulated by phosphorylation, as indicated by its inclusion in phosphoproteomic studies.

Experimental Guidance and Technical Tips

• The antibody is generated against a recombinant fusion protein spanning amino acids 343–622 of human FANCG. In Western blot, a band corresponding to the predicted molecular weight of approximately 68–69 kDa may be observed; however, validation in the relevant sample system (e.g., human or mouse cell lysates) is recommended.
• For ELISA, the antibody may be suitable as a coating or detection reagent; empirical optimization of antibody concentration and assay conditions is advised.
• Since this is a polyclonal antibody, lot-to-lot consistency in affinity and specificity may vary; consider performing titration experiments or pre-adsorption controls for critical applications.
• Cross-reactivity with mouse FANCG has been reported, suggesting utility in murine models; confirm by sequence alignment and experimental validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a reliable supply chain for high-quality IVD raw materials, including this FANCG polyclonal antibody for DNA repair and Fanconi anemia studies. Beyond antibodies, we offer validated antibody pairs, bulk ancillary reagents, and customized sourcing of rare targets to streamline your assay development from concept to clinic.