Anti-FANCD2 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q9BXW9

Core Product Specifications and Parameters

Biological Background: FANCD2 Function and Localization

• FANCD2 (Fanconi anemia group D2 protein) is encoded by the FANCD2 gene and is also known as FA4, FAD, FACD, FAD2, FA-D2, and FANCD. Alternative splicing yields multiple isoforms (UniProt Q9BXW9).

• FANCD2 is required for the maintenance of chromosomal stability and promotes accurate and efficient homolog pairing during meiosis. Related references: PMID:11239453 PMID:14517836

• The protein is involved in the repair of DNA double-strand breaks via homologous recombination and single-strand annealing, and the FANCI-FANCD2 complex scans dsDNA for damage, stalling at dsDNA-ssDNA junctions. Related references: PMID:15671039 PMID:15650050 PMID:30335751

• FANCD2 may activate S-phase and G2-phase checkpoints upon DNA damage, helping to coordinate cell cycle progression with repair. Related references: PMID:15377654

• It prevents breakage and loss of missegregating chromatin after replication stress and is required for targeting/stabilizing BLM to abnormal structures induced by replicative stress. Related references: PMID:15454491 PMID:15661754 PMID:19465921

• FANCD2 promotes BRCA2/FANCD1 loading onto damaged chromatin, facilitating DNA repair by homologous recombination. Related references: PMID:11239454 PMID:12239151 PMID:12086603

• The protein localizes to the nucleus and is highly expressed in germinal center cells of spleen, tonsil, reactive lymph nodes, proliferating basal epithelial layers, cytotrophoblastic cells, and testicular spermatocytes.

• As a Fanconi anemia protein, FANCD2 is associated with a rare inherited bone marrow failure syndrome and cancer predisposition; disease-causing variants have been mapped to this gene.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 540–720 of human FANCD2, a central region that is likely conserved across isoforms; consider verifying reactivity with your specific sample type.
• The antibody has been validated by the supplier for WB, IF/ICC, IP, and ELISA. In WB, a band near 164 kDa is expected; validate this in your system using appropriate positive and negative controls.
• Reported cross-reactivity includes human and rat; if using in other species, confirm sequence homology and validate experimentally.
• For IF/ICC, anticipate nuclear localization; include DNA counterstaining to confirm target compartment.
• For IP and ELISA, the polyclonal nature may enhance signal but lot-to-lot consistency should be verified for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support FANCD2-related DNA repair and Fanconi anemia research and diagnostic development. Backed by comprehensive technical services and consulting, we empower your transition from concept to clinic.