Anti-FANCD2 Rabbit Monoclonal Antibody for WB, IP, ELISA - Q9BXW9

Core Product Specifications and Parameters

Biological Background: FANCD2 Function and Localization

• FANCD2 is required for maintenance of chromosomal stability. Related references: PMID:11239453 PMID:14517836
• It promotes accurate and efficient pairing of homologs during meiosis. Related references: PMID:14517836
• Involved in the repair of DNA double-strand breaks by homologous recombination and single-strand annealing. Related references: PMID:15671039 PMID:15650050 PMID:30335751
• The FANCI-FANCD2 complex binds and scans dsDNA for damage, stalling at DNA junctions between dsDNA and ssDNA.
• May participate in S phase and G2 phase checkpoint activation upon DNA damage. Related references: PMID:15377654
• Plays a role in preventing breakage and loss of missegregating chromatin at the end of cell division, particularly after replication stress. Related references: PMID:15454491 PMID:15661754
• Required for the targeting or stabilization of BLM to non-centromeric abnormal structures induced by replicative stress. Related references: PMID:15661754 PMID:19465921
• Promotes BRCA2/FANCD1 loading onto damaged chromatin. Related references: PMID:11239453 PMID:12239151 PMID:12086603
• Localizes to the nucleus and is highly expressed in germinal center cells, testis spermatocytes, and proliferating epithelial cells.
• Keywords: Alternative splicing, Cell cycle, Disease variant, DNA damage, DNA repair, Fanconi anemia, Isopeptide bond, Nucleus, Phosphoprotein, Reference proteome, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning residues 200–300 of human FANCD2, suggesting the antibody may recognize an epitope within this region. Consider using nuclear-enriched lysates for Western blot to improve signal.
• The predicted molecular weight is ~164 kDa. Use an appropriate gel percentage (e.g., 6–8% SDS-PAGE) and transfer conditions for high-molecular-weight proteins.
• For IP, validate antibody performance under native conditions with relevant controls. For ELISA, confirm binding using the immunogen peptide or recombinant FANCD2.
• Cross-reactivity is reported for human, mouse, and rat; verify specificity in your sample system using knockout or knockdown controls if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive sourcing solution for IVD raw materials, including validated monoclonal antibodies like this anti-FANCD2 rabbit mAb, optimized for DNA damage and Fanconi anemia pathway analysis. From bulk antibody pairs and optimized ancillary reagents to rare-target sourcing, our platform supports your assay development needs from concept to clinic.