Anti-FANCC Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q00597

Core Product Specifications and Parameters

Biological Background: FANCC Function and Localization

• Encodes the Fanconi anemia group C protein, a key component of the Fanconi anemia (FA) pathway involved in DNA interstrand cross-link repair.
• Functions in postreplication repair and cell cycle checkpoint control, essential for maintaining chromosome stability upon DNA damage.
• Upon interferon-gamma (IFNG) stimulation, facilitates STAT1 activation by recruiting STAT1 to the IFNGR1 receptor complex.
• Localizes to both the nucleus and cytoplasm, consistent with its dual roles in DNA repair and signal transduction.
• Ubiquitously expressed across human tissues, reflecting its fundamental role in genomic integrity.
• Classified under keywords including 'DNA damage', 'DNA repair', 'Fanconi anemia', and 'Disease variant', linking mutations to the cancer-prone disorder Fanconi anemia.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 340-558), which may be suitable for detecting full-length FANCC across species due to conservation.
• For Western blot (WB), the predicted molecular weight is 63 kDa; use appropriate gel percentage and transfer conditions for mid-sized proteins.
• In immunohistochemistry (IHC-P), consider antigen retrieval protocols and validate staining patterns in known FANCC-expressing tissues.
• For immunofluorescence (IF/ICC), fixation and permeabilization may affect detection; test with appropriate controls to distinguish cytoplasmic and nuclear localization.
• ELISA can be used for quantitative detection; consider pairing with capture antibodies for sandwich ELISA development.
• Validate species cross-reactivity in mouse and rat samples, as sequence homology in the immunogen region may influence detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in sourcing high-quality IVD raw materials, including validated antibody pairs and recombinant proteins for DNA damage repair and cancer research. This anti-FANCC polyclonal antibody exemplifies our commitment to providing reliable tools for Fanconi anemia and genomic stability studies, supported by bulk supply options and custom development services.