Anti-FAM98B Rabbit Polyclonal Antibody for WB and ELISA - Q52LJ0

Core Product Specifications and Parameters

Biological Background: FAM98B Function and Localization

• FAM98B is a subunit of the tRNA-splicing ligase complex, essential for maturation of tRNAs by directly ligating tRNA halves. Related references: PMID:21311021 PMID:24870230
• It functions by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester bond, suggesting a key role in RNA processing. Related references: PMID:21311021 PMID:24870230
• FAM98B may also act as a broad-specificity RNA ligase and regulate the expression of the protein arginine N-methyltransferase PRMT1. Related references: PMID:28040436
• Subcellular localization studies indicate that FAM98B is present in both the nucleus and the cytoplasm.
• At the protein level, FAM98B is highly expressed in colorectal cancer tissues compared to normal colon, highlighting its potential as a cancer biomarker or therapeutic target. Related references: PMID:28040436
• The protein is characterized by keywords including alternative splicing, cytoplasm, nucleus, proteomics identification, and tRNA processing, reflecting its multifunctional nature.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1–225) of human FAM98B; the antibody may recognize full-length and N-terminal fragments or isoforms.
• For Western blot (WB), the expected band is approximately 46 kDa; validate the antibody in your specific sample matrix and consider additional bands from post-translational modifications or alternative splicing events.
• This polyclonal antibody is suitable for ELISA applications; as a polyclonal, it offers broad epitope coverage—consider it for use as both capture and detection reagent in sandwich assays.
• Cross-reactivity with mouse and rat has been reported; perform validation in the relevant species and tissue type before quantitative experiments.
• Always include appropriate negative and positive controls, and verify antibody performance under your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your diagnostic development by providing a comprehensive range of validated IVD raw materials, including monoclonal and polyclonal antibodies, antibody pairs, and ancillary reagents. For targets like FAM98B, involved in tRNA processing and colorectal cancer, CamelBio offers tailored sourcing solutions to accelerate your assay design and verification. From concept to clinic, our integrated supply and technical consulting services support reliable, scalable manufacturing of high-performance diagnostic kits.