Anti-FAM96B Polyclonal Antibody for WB, ELISA - Q9Y3D0

Core Product Specifications and Parameters

Biological Background: FAM96B Function and Localization

• FAM96B, also known as CIA2B, MIP18, or CGI-128, is a conserved 163 amino acid protein with a predicted mass of approximately 18 kDa that functions as a component of the cytosolic iron-sulfur protein assembly (CIA) machinery.
• As a core CIA complex member together with CIAO1 and MMS19, FAM96B participates in the biogenesis and delivery of Fe-S clusters to numerous cytosolic and nuclear client proteins. Related references: PMID:22678361, PMID:22678362, PMID:23891004, PMID:29848660
• In the mitotic spindle-associated MMXD complex, FAM96B contributes to proper chromosome segregation by facilitating Fe-S cluster insertion into the DNA repair helicase ERCC2/XPD. Related references: PMID:20797633
• Together with MMS19, FAM96B directly mediates Fe-S cluster transfer to the kinesin motor protein KIF4A, ensuring its correct localization to mitotic structures and promoting normal progression of mitosis. Related references: PMID:29848660
• Subcellularly, FAM96B localizes to both the nucleus and cytoplasm, and is enriched at the mitotic spindle and midbody, consistent with its dual roles in Fe-S cluster assembly and chromosome dynamics.
• Alternative names reflect distinct functional contexts: CIA2B denotes its CIA machinery role, MIP18 highlights its involvement in mitosis, and FAM96B/CGI-128 are historical gene designations.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to amino acids 1–163 of human FAM96B, covering the full-length protein; this design may recognize both human and mouse orthologs due to high sequence conservation.
• For Western blotting, consider using a protein extract from human or mouse cell lines expressing FAM96B and expect a band near 18 kDa; loading controls and proper blocking conditions should be optimized.
• In ELISA applications, the antibody can be used to detect recombinant or endogenous FAM96B; assay linearity and detection limits should be validated with appropriate standard curves.
• Because FAM96B is involved in dynamic processes such as mitosis and Fe-S cluster assembly, treatment conditions (e.g., cell cycle synchronization) may affect protein levels; inclusion of relevant controls is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop supply of IVD raw materials like validated antibodies and ancillary reagents. For studies focusing on cytosolic iron-sulfur cluster assembly and mitotic regulation, this FAM96B polyclonal antibody offers a reliable tool, and CamelBio can also source complementary reagents and custom antibody pairs to streamline your workflow.