Anti-FAM65B Rabbit Polyclonal Antibody for WB, IF-P, ELISA - Q9Y4F9

Core Product Specifications and Parameters

Biological Background: FAM65B (RIPOR2) Function and Localization

• FAM65B (also known as RIPOR2, C6orf32, DIFF48, PL48) is a Rho family-interacting cell polarization regulator 2 that inhibits the small GTPase RHOA, key to cytoskeletal dynamics.
  Related references:
  PMID:17150207 PMID:23241886 PMID:24687993
• It promotes filopodia and myotube formation during fetal mononuclear myoblast differentiation, linking it to myogenesis.
  Related references:
  PMID:17150207
• Essential for hearing: required for normal development of cochlear inner and outer hair cell stereocilia, and maintaining the basal domain of stereocilia.
  Related references:
  PMID:24958875
• Negatively regulates T lymphocyte responses: inhibits chemokine-induced adhesion, polarization, and migration, keeping naïve T cells quiescent.
  Related references:
  PMID:23241886 PMID:27556504
• Subcellular localization includes cytoplasm, cytoskeleton, filopodia, stereocilia membrane, and apical cell membrane, consistent with its role in cellular protrusions.
• Expressed in primary fetal myoblasts, naïve T lymphocytes (high), activated T cells (low), blood cells, secondary lymphoid organs, and cytotrophoblast.
  Related references:
  PMID:17150207 PMID:27556504 PMID:23241886 PMID:9055809
• Phosphoprotein and alternatively spliced; mutations in RIPOR2 cause non-syndromic deafness DFNA21 and DFNB104.
• Keywords: cell adhesion, chemotaxis, deafness, myogenesis, hearing, differentiation, developmental protein.

Experimental Guidance and Technical Tips

• For Western blotting (WB), the predicted molecular weight is ~119 kDa; use appropriate gel percentage (e.g., 8% SDS-PAGE) and wet transfer conditions. Validate detection with positive control lysates from human, mouse, or rat tissues expressing FAM65B (e.g., lymphoid organs, muscle cells).
• For immunofluorescence/immunocytochemistry (IF-P), optimized fixation and permeabilization protocols are necessary to visualize FAM65B in filopodia, stereocilia, or cytoplasmic compartments. Perform appropriate isotype controls.
• For ELISA, pair with a suitable detection antibody and validate linearity and specificity using recombinant FAM65B protein or cell lysates.
• The antibody was raised against the N-terminal 1-210 amino acid region. Because alternative splicing may generate isoforms, confirm band pattern or localization in your model system by siRNA knockdown or CRISPR knockout controls.
• Cross-reactivity is reported for human, mouse, and rat; sequence homology should be checked for other species before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined pathway from target identification to IVD assay development, supplying high-quality raw materials like this anti-FAM65B polyclonal antibody. For researchers investigating cell polarization, hearing disorders, or myogenesis, CamelBio can provide matching validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our sourcing capabilities extend to rare targets, ensuring consistent supply for diagnostic manufacturing and translational research.