Anti-FAM50B Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9WTJ8

Core Product Specifications and Parameters

Biological Background: FAM50B Function and Localization

• Protein FAM50B, also known as XAP-5-like, is encoded by the Fam50b gene (also designated D0H6S2654E or X5l) in mouse.
• Tissue expression analysis reveals a wide distribution, with prominent expression in testis. Within the testis, FAM50B is detected in seminiferous tubules, specifically in primary spermatocytes and round spermatids, but is absent from spermatogonia, elongating spermatids, mature spermatozoa, Sertoli cells, and Leydig cells.
• Annotated with the keyword 'Acetylation', indicating that the protein is subject to post-translational acetylation modification.
• Included in the UniProt Reference proteome set, signifying a manually reviewed and representative sequence for Mus musculus.
• The calculated molecular weight from the sequence is 39594 Da; the observed band on western blot is approximately 40 kDa.
• No specific molecular function or biological process has been assigned to FAM50B in the reviewed UniProt record.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 70–150 of mouse FAM50B, a region that may represent a unique epitope; consider verifying specificity using knockout or knockdown models if available.
• For western blot (WB), a start dilution range of 1:500–1:2000 is common for rabbit polyclonal antibodies; confirm the optimal dilution with your sample lysates.
• Testis tissue lysates from human, mouse, or rat may serve as positive controls due to the abundant expression observed in seminiferous tubules.
• In immunohistochemistry (IHC-P), antigen retrieval conditions may influence staining; consider testing both heat-induced and enzymatic retrieval methods on testis sections.
• The antibody is also validated for ELISA by the supplier; use the provided immunogen or cell lysates to develop your assay.
• Always include appropriate negative controls (e.g., pre-immune serum or omission of primary antibody) in your experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research laboratories with validated antibodies, bulk reagents, and rare-target raw materials. For FAM50B research—which may involve testis biology, acetylation studies, or antibody development—CamelBio facilitates access to high-quality rabbit polyclonal antibodies like this one and can assist in sourcing ancillary reagents and custom antibody pairs to accelerate your assay development. With a commitment to end-to-end support from concept to clinic, CamelBio is your reliable bridge for critical raw material procurement.