Anti-FAM46A Polyclonal Antibody for WB, ELISA - Q96IP4

Core Product Specifications and Parameters

Biological Background: FAM46A Function and Localization

• FAM46A (Terminal nucleotidyltransferase 5A, TENT5A), also known as HBV X-transactivated gene 11 protein, is encoded by the TENT5A gene and belongs to the nucleotidyltransferase family.
• Functions as a cytoplasmic non-canonical poly(A) RNA polymerase that catalyzes the addition of an adenosine molecule from ATP to the 3'-OH end of mRNA poly(A) tails, thereby participating in cytoplasmic polyadenylation. Related references: PMID:33882302
• Plays a key role in bone mineralization by polyadenylating mRNAs encoding extracellular matrix constituents and other genes crucial for osteoblast function, with a preference for ER-targeted mRNAs.
• Localizes predominantly to the cytoplasm, where it regulates mRNA poly(A) tail length and translational efficiency.
• Widely expressed across tissues, with notably higher expression in the retina and osteoblasts. Related references: PMID:12054608 PMID:29358272
• Annotated with keywords including alternative splicing, disease variant, and osteogenesis imperfecta, reflecting its clinical relevance in skeletal disorders.
• The protein has a predicted molecular weight of approximately 50 kDa, aligning with the 49666 Da theoretical mass.

Experimental Guidance and Technical Tips

• For Western blot, a band near 50 kDa is expected; consider using whole-cell lysates from osteoblast or retina cell lines as positive controls, and validate antibody specificity with siRNA knockdown or knockout samples.
• In ELISA applications, the recombinant immunogen (amino acids 180-400) may serve as a suitable coating antigen or standard; titrate antibody concentration to achieve optimal signal-to-noise ratio.
• The antibody cross-reacts with human and mouse TENT5A; verify reactivity in the species and sample type of interest, and note that additional species may require experimental confirmation.
• When probing FAM46A expression, be aware of its cytoplasmic localization and potential expression variability across tissues; include appropriate loading controls for normalization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories seamless access to validated IVD raw materials, including this FAM46A polyclonal antibody for bone mineralization and osteogenesis studies. As a comprehensive sourcing partner, we provide optimized antibody pairs, bulk ancillary reagents, and custom procurement solutions for rare targets, accelerating your transition from concept to clinic. Rely on CamelBio for consistent, high-quality immunoreagents tailored to your assay development needs.