Anti-FAM3D Polyclonal Antibody for WB, ELISA - Q96BQ1

Core Product Specifications and Parameters

Biological Background: Protein FAM3D Function and Localization

• Protein FAM3D, also known as EF7 or OIT1, is a secreted glycoprotein belonging to the FAM3 family.
• It is encoded by the FAM3D gene and is annotated as a lectin, suggesting potential carbohydrate-binding activity.
• The protein possesses a signal peptide and a disulfide bond motif, consistent with its secretion pathway.
• Subcellular localization: Secreted into the extracellular space.
• Tissue specificity: Abundantly expressed in the placenta, with weak expression in the small intestine.
• Molecular weight: Predicted mass of approximately 25 kDa (24963 Da), consistent with a 25 kDa band observed in Western blot.
• Functional characterization remains limited; however, its expression pattern and secreted nature imply possible roles in intercellular signaling or immune modulation.
• Keywords: Disulfide bond, Glycoprotein, Lectin, Proteomics identification, Reference proteome, Secreted, Signal.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 24-204 of human FAM3D (NP_620160.1), a region that likely excludes the N-terminal signal peptide, targeting the mature secreted form. Consider this when designing detection strategies.
• Recommended applications: Western blot (WB) and enzyme-linked immunosorbent assay (ELISA). For WB, a band around 25 kDa is expected under reducing conditions; validate in relevant sample systems (e.g., placental tissue lysates, conditioned media).
• Cross-reactivity is reported with mouse and rat, enabling multi-species studies. Validate in the respective species before quantitative assays.
• As a polyclonal antibody, lot-to-lot consistency should be verified, and optimal working dilutions should be determined empirically for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supplying high-quality polyclonal antibodies like this anti-FAM3D antibody for diagnostic assay development. Our portfolio includes validated antibody pairs, monoclonal and polyclonal options, bulk ancillary reagents, and custom sourcing for rare targets. Whether you are investigating placental biology or developing ELISA kits targeting secreted glycoproteins, CamelBio bridges your needs from concept to clinic with reliable raw-material supply.