Anti-FAM3B Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P58499

Core Product Specifications and Parameters

Biological Background: FAM3B Function and Localization

• FAM3B, also known as pancreatic-derived factor (PANDER) or cytokine-like protein 2-21, is a secreted cytokine-like protein.
• It induces apoptosis of alpha and beta cells in a dose- and time-dependent manner.
• FAM3B is highly expressed in the pancreas, with additional expression in the colon, kidney, prostate, small intestine, and testis.
• The protein contains a signal peptide, is secreted, and features disulfide bonds and glycosylation.
• Alternative splicing generates multiple isoforms of FAM3B.
• It belongs to the FAM3 gene family and has been identified as a lectin.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 30–235, which corresponds to the secreted portion of the protein; consider using this antibody to detect endogenous FAM3B in pancreatic tissue or cell culture supernatants.
• For WB, the predicted molecular weight is ~26 kDa, but glycosylation may result in a higher apparent mass; validate band specificity using appropriate controls.
• IHC-P can be performed on pancreas sections to examine expression in islet cells; antigen retrieval conditions may require optimization.
• IF/ICC may reveal subcellular localization patterns in pancreatic cell lines; co-staining with islet cell markers is recommended.
• ELISA can be considered for quantifying secreted FAM3B if the antibody pair is validated; cross-reactivity with mouse and rat expands utility to rodent models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality IVD raw materials, such as the FAM3B rabbit polyclonal antibody, supporting apoptosis and pancreatic disease research. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to help you accelerate your diagnostic development from concept to clinic.