Anti-Fam221a Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8C790

Core Product Specifications and Parameters

Biological Background: Fam221a Function and Localization

• FAM221A (Family with sequence similarity 221 member A) is a protein encoded by the Fam221a gene in mouse, with human and rat orthologs identified.
• The protein is part of the Mus musculus reference proteome, representing a well-annotated canonical sequence.
• Alternative splicing of the Fam221a transcript generates multiple isoforms, though their functional significance remains unclear.
• The predicted molecular weight of the canonical isoform is approximately 33 kDa, consistent with the observed 33kDa band in experimental settings.
• Subcellular localization and biological function of FAM221A are not yet characterized; no conserved functional domains have been reported.
• The immunogen used for this antibody corresponds to the N-terminal region (aa 1-292), covering most of the protein sequence and enabling detection of full-length FAM221A.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-292 of mouse Fam221a, so the antibody is expected to recognize N-terminal epitopes; consider this when analyzing truncated or isoform-specific signals.
• For Western blotting, start with a standard dilution range (e.g., 1:500–1:2000) and adjust based on signal intensity and background in your specific lysates.
• In immunofluorescence/immunocytochemistry, optimize fixation and permeabilization protocols for the target cell line to preserve antigenicity and accessibility.
• ELISA applications may benefit from testing a dilution series of both the antibody and antigen to establish a robust standard curve.
• Cross-reactivity with human and rat is indicated; verify band patterns or staining in relevant species-derived samples to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material procurement partner, providing diagnostic manufacturers and research labs with validated antibodies, bulk ancillary reagents, and rare target sourcing. This Fam221a polyclonal antibody supports your mouse model studies and cross-species applications; reach out to explore tailored supply solutions including antibody pairs and optimized monoclonal/polyclonal reagents. Let CamelBio streamline your supply chain from concept to clinic.