Anti-FAM20A Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q96MK3

Core Product Specifications and Parameters

Biological Background: FAM20A Function and Localization

• FAM20A is a pseudokinase that acts as an allosteric activator of the Golgi-resident serine/threonine kinase FAM20C, essential for enamel matrix protein phosphorylation.
• It forms a functional complex with FAM20C, enhancing the kinase activity of FAM20C toward proteins involved in biomineralization of teeth.
• FAM20A is implicated in amelogenesis imperfecta, a disorder of dental enamel formation; disease-associated mutations underscore its critical role in enamel biomineralization.
• Subcellularly, FAM20A localizes to the endoplasmic reticulum and Golgi apparatus, and is also secreted, consistent with its role in modifying extracellular matrix proteins.
• Tissue expression profiling reveals high levels in lung and liver, with intermediate expression in thymus and ovary, suggesting potential extra-skeletal functions.
• The protein contains a signal peptide, is glycosylated, and has predicted disulfide bonds, features typical of secreted and Golgi-resident proteins.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a specific peptide region (aa 140-240), so antibody reactivity is expected to be target-specific; however, polyclonal antibodies may recognize multiple epitopes.
• For Western blot, the predicted molecular weight is 61 kDa; consider using lung or liver tissue lysates, where FAM20A is highly expressed.
• In immunohistochemistry-paraffin (IHC-P), antigen retrieval conditions and tissue fixation may affect epitope accessibility; positive control tissues include lung and liver sections.
• For ELISA, pairing with a monoclonal detection antibody may improve specificity; validate the assay using recombinant FAM20A protein if available.
• Always include appropriate no-primary-antibody and isotype controls to distinguish specific from non-specific signals.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering a comprehensive portfolio including validated antibody pairs, bulk ancillary reagents, and optimized polyclonal antibodies for targets like FAM20A. We support diagnostic developers and researchers with seamless access to high-quality products from concept to clinic, backed by expert technical consultation. For your dental enamel and biomineralization research needs, CamelBio can also assist in rare target raw-material procurement and custom assay development.