Anti-FAM20A Monoclonal Antibody for WB - Q96MK3

Core Product Specifications and Parameters

Biological Background: FAM20A Function and Localization

• FAM20A (family with sequence similarity 20 member A) is a pseudokinase that acts as an allosteric activator of the Golgi kinase FAM20C.
• It forms a complex with FAM20C, enhancing the phosphorylation of secretory calcium-binding phosphoproteins that constitute the enamel matrix, thereby playing a critical role in tooth biomineralization.
• The protein is localized to the Golgi apparatus and endoplasmic reticulum, and is also secreted.
• FAM20A is highly expressed in lung and liver, with moderate expression in thymus and ovary.
• It contains disulfide bonds and is glycosylated, indicative of its secretory pathway processing.
• Mutations in FAM20A are associated with amelogenesis imperfecta and gingival fibromatosis syndrome, highlighting its importance in dental development.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within the C-terminal region (aa 442-541), which may be suitable for detecting full-length FAM20A in Western blotting.
• For ELISA, antigen coating with recombinant protein or peptide may be considered to optimize detection.
• Due to its association with the Golgi/ER, sample preparation with subcellular fractionation or denaturing lysis buffers may improve detection.
• Cross-reactivity has been validated for human; reactivity with other species is unknown and should be verified if non-human samples are used.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, including validated monoclonal antibodies like this FAM20A antibody, optimized for dental and mineralization research. Beyond antibodies, we offer bulk ancillary reagents and custom sourcing for rare target antigens to support your diagnostic development from concept to clinic.