Anti-FAM173B Polyclonal Antibody for WB and ELISA - Q6P4H8

Core Product Specifications and Parameters

Biological Background: ATP synthase subunit C lysine N-methyltransferase (FAM173B) Function and Localization

• FAM173B (ATPSCKMT) encodes a mitochondrial protein-lysine N-methyltransferase that trimethylates ATP synthase subunit C (ATP5MC1/ATP5MC2), essential for ATP synthase complex assembly and mitochondrial respiration. Related references: PMID:29444090 PMID:30530489
• Plays a critical role in chronic pain: its methyltransferase activity in sensory neuron mitochondria drives persistent inflammatory and neuropathic pain through reactive oxygen species production and spinal cord microglia activation. Related references: PMID:29444090
• Localizes to the mitochondrial membrane and is ubiquitously expressed across human tissues.
• Contains transmembrane helices and a S-adenosyl-L-methionine-dependent methyltransferase domain.
• The protein undergoes acetylation and alternative splicing, generating multiple isoforms.
• Part of a family of protein N-lysine methyltransferases implicated in diverse nuclear and mitochondrial regulatory processes.

Experimental Guidance and Technical Tips

• The immunogen comprises the full-length recombinant FAM173B protein (amino acids 1–233); this broad sequence coverage may enhance detection of multiple isoforms and variants in Western blotting.
• Recommended species include human, mouse, and rat as per supplier‑indicated cross‑reactivity; endogenous expression should be verified in target cell lines or tissue lysates.
• For ELISA applications, consider pairing with a validated detection antibody and optimizing coating conditions using recombinant protein standards.
• It is advisable to confirm antibody specificity by performing a blocking experiment with the recombinant immunogen or by siRNA/shRNA knockdown in a suitable model.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we provide a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents, to support diagnostic manufacturers and research laboratories. This anti‑FAM173B antibody, targeting a mitochondrial methyltransferase linked to chronic pain and metabolic regulation, is available through our one‑stop sourcing platform, together with rare‑target raw material procurement and technical consulting. Let CamelBio accelerate your assay development from concept to clinic with reliable, scalable supply solutions.