Anti-FAM120A Rabbit Polyclonal Antibody for WB, ELISA - Q9NZB2

Core Product Specifications and Parameters

Biological Background: FAM120A Function and Localization

• FAM120A (Constitutive coactivator of PPAR-gamma-like protein 1, also known as OSSA or C9orf10) is a membrane-associated scaffold protein that participates in oxidative stress-induced survival signaling, RNA trafficking, and translational regulation. Related references: PMID:19015244
• It acts as an upstream regulator of intracellular kinases SRC, FAK, and PI3K following oxidative stress, promoting cell survival. Related references: PMID:19015244
• FAM120A promotes RNA splicing and stability of lipogenic enzymes by recruiting the transcription factor SREBP1 and bridging nascent transcripts to the SRSF1/U1-70K splicing machinery. Related references: PMID:37595559
• It associates with NLRP3 inflammasome components and inhibits NLRP3 inflammasome activation. Related references: PMID:40250624
• FAM120A can sequester a subset of AGO2-bound mRNAs from miRNA-mediated silencing by binding to poly(G)-rich motifs in their 3′-UTR, likely preventing recruitment of miRISC effectors.
• Subcellularly, FAM120A localizes predominantly to the cytoplasm and cell membrane.
• It is widely expressed, and in gastric mucosa, detected in the bottom region of the foveolar epithelium. Related references: PMID:14585507, PMID:19015244
• Post-translational modifications include acetylation, methylation, and phosphorylation; it is classified as an RNA-binding protein and a potential tumor suppressor.

Experimental Guidance and Technical Tips

• The immunogen used comprises the N-terminal 140 amino acids of human FAM120A; consider this when analyzing domain-specific interactions or isoforms.
• For Western blot, an expected band around 122kDa should be observed; include appropriate positive and negative controls to validate specificity.
• For ELISA, the recombinant protein immunogen can be used as coating antigen; however, optimal working dilutions should be determined empirically.
• Given the protein’s membrane association and involvement in stress signaling, consider validating the antibody in relevant cellular models or stimulation conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. Our expertise in the FAM120A signaling and metabolic pathway field supports diagnostic manufacturers with reliable reagents, technical services, and consulting—from concept to clinical application.