Anti-FAK Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q05397

Core Product Specifications and Parameters

Biological Background: FAK Function and Localization

• Protein identity: FAK (Focal adhesion kinase 1), also known as PTK2, is a non-receptor protein-tyrosine kinase that serves as a critical regulator of cell adhesion, migration, and cytoskeletal reorganization. It exists in multiple isoforms, including full-length FAK and an inhibitory isoform FRNK (FAK-related non-kinase) that lacks the kinase domain.
• Core functions: FAK transduces signals from integrins and growth factor receptors, coordinating focal adhesion dynamics, cell spreading, and motility. It forms signaling complexes with SRC family kinases, leading to phosphorylation of downstream effectors like paxillin and CAS family members (PubMed:9360983).
  Related references:
  PMID:9360983
• Gene family: Encoded by the PTK2 gene, FAK belongs to the FAK family of tyrosine kinases. It is ubiquitously expressed, with prominent roles in embryonic development, angiogenesis, and nervous system formation.
• Subcellular localization: FAK localizes primarily to focal adhesions at the cell membrane and is also found in the cytoplasm, nucleus, centrosome, and cilium basal body. Its shuttling between adhesion sites and the nucleus allows integration of mechanical and growth signals.
• Post-translational modifications and keywords: FAK undergoes autophosphorylation at Y397, creating a docking site for SRC. Additional phosphorylation, acetylation, and ubiquitination regulate its activity and stability. Keywords include ATP-binding, cell junction, kinase, phosphoprotein, and developmental protein.
• Tissue specificity: Ubiquitously expressed in various tissues, FAK is detected in B and T lymphocytes, lung fibroblasts, and epithelial cells (PubMed:31630787). Its expression is essential for normal cardiac and neural development.
  Related references:
  PMID:31630787

Experimental Guidance and Technical Tips

• The immunogen (amino acids 734–834) corresponds to the C-terminal focal adhesion targeting (FAT) domain, which is present in both full-length FAK and the FRNK isoform. Therefore, the antibody may detect both forms—consider validating in your cell/tissue model with appropriate molecular weight markers (full-length ~119 kDa, FRNK ~41 kDa).
• For western blotting, ensure sufficient protein loading and optimize primary antibody dilution; a band at 119 kDa is expected for full-length FAK in most human, mouse, and rat samples.
• In immunohistochemistry and immunofluorescence, FAK typically concentrates at focal adhesions near the cell periphery, but may also show cytoplasmic or perinuclear staining depending on cell type and activation state. Use appropriate fixation and permeabilization methods.
• For immunoprecipitation, the polyclonal nature may provide robust capture; confirm with positive control lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, CROs, and research labs streamlined access to IVD raw materials—from validated monoclonal and polyclonal antibodies to bulk ancillary reagents. This anti-FAK antibody is part of our portfolio supporting cell adhesion, migration, and oncology research. Rely on us for one-stop sourcing of antibody pairs, rare-target reagents, and technical consultation to accelerate your assay development.