Anti-FAK (Focal Adhesion Kinase) Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q05397

Core Product Specifications and Parameters

Biological Background: FAK Function and Localization

• Focal adhesion kinase 1 (FAK) is a non-receptor protein-tyrosine kinase essential for regulating cell migration, adhesion, spreading, actin cytoskeleton reorganization, and focal adhesion dynamics. It also participates in cell cycle progression, proliferation, and apoptosis.
• FAK is required for early embryonic development, placenta formation, angiogenesis, and normal development of the heart and nervous system, including axon growth and synapse formation.
• Upon activation, FAK forms multisubunit signaling complexes with SRC and SRC family members, leading to phosphorylation of additional tyrosine residues that serve as docking sites for scaffold proteins, effectors, and substrates, thereby modulating pathways such as PI3K/AKT and MAPK.
• FAK phosphorylates NEDD9 following integrin stimulation, contributing to the activation of RAC1 through CAS family members. Related references: PMID:9360983
• The protein localizes to multiple subcellular compartments, including focal adhesions, cell membrane, perinuclear cytoplasm, cell cortex, centrosome, nucleus, and cilium basal body, reflecting its dynamic role in signal transduction and mechanotransduction.
• Isoform 6 (FRNK) lacks the kinase domain and acts as a dominant-negative inhibitor of FAK phosphorylation and signaling, attenuating nuclear accumulation of LPXN and limiting serum response factor (SRF)-dependent gene transcription.
• FAK is ubiquitously expressed and detected in B and T-lymphocytes, lung fibroblasts, and epithelial cells. Related references: PMID:31630787
• Belongs to the protein kinase superfamily, Tyr protein kinase family, and is subject to various post-translational modifications including phosphorylation, acetylation, and ubiquitin conjugation, which regulate its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen sequence (amino acids 700-800) lies outside the kinase domain; this antibody may recognize both full-length FAK and the FRNK isoform, offering flexibility for detecting total FAK expression.
• In WB, expect a band near 119 kDa; consider using appropriate protein extraction and loading controls to monitor FAK levels across different cell types or conditions.
• For IF/ICC, FAK localizes prominently to focal adhesions; optimize fixation and permeabilization protocols to preserve these structures and validate staining with suitable controls in your sample system.
• Cross-reactivity with mouse and rat samples is predicted; verify performance in your model organism to ensure specificity and sensitivity.
• Antibody performance in ELISA may require optimization of coating antigen and detection conditions; pairing with a validated capture/detection antibody may enhance signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with verified IVD raw materials, including this FAK monoclonal antibody for cell adhesion and migration studies. As a comprehensive partner, we offer validated antibody pairs, monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your assay development from concept to clinical application.