Anti-FAIM3 Rabbit Polyclonal Antibody for WB, ELISA - O60667

Core Product Specifications and Parameters

Biological Background: FAIM3 Function and Localization

• FAIM3 (also known as TOSO, FcmuR) encodes the immunoglobulin mu Fc receptor, a high-affinity receptor for both secreted and membrane-bound IgM. This receptor primarily regulates IgM transport, homeostasis, and effector functions in B cell development and primary immune responses. Related references: PMID:19858324, PMID:22675200, PMID:36949194

• The protein mediates bidirectional trafficking of IgM: it enables exocytosis of membrane-bound IgM on the plasma membrane and endocytosis of IgM-antigen complexes toward lysosomes for degradation. In mucosal epithelium, FAIM3 facilitates retrotranscytosis of antigen-IgM complexes across M cells to antigen-presenting cells in mucosal lymphoid tissues. Related references: PMID:21908732, PMID:28230186

• FAIM3 triggers costimulatory signaling via the non-canonical NF-kappa-B, MAP kinases, and calcium signaling pathways, thereby modulating tonic BCR signaling and supporting negative selection of autoreactive B cells. It also promotes induction of an efficient neutralizing IgG response to both T cell-dependent and -independent antigens. Related references: PMID:19858324, PMID:22675200, PMID:25601920

• The receptor is localized to the cell membrane, early endosome membrane, Golgi apparatus, trans-Golgi network membrane, and lysosome membrane. A secreted form also exists, suggesting dynamic trafficking and potential soluble receptor functions.

• Tissue expression is predominantly in CD19-positive B cells and CD4/CD8-positive T cells in primary and secondary lymphoid organs. At the protein level, it is detected in bone marrow pro-/pre-B cells, follicular and memory B cells, and a small subset of germinal center B cells, as well as lower levels in NK cells. Transcripts are also found in lymph nodes, lung, thymus, and kidneys.

• Keywords highlight structural features including an immunoglobulin domain, disulfide bonds, phosphorylation sites, and alternative splicing, along with its classification as a transmembrane receptor involved in immunity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 18–251 of the human FAIM3 extracellular domain; consider using membrane-enriched protein preparations for optimal detection in Western blot.
• Predict a band at approximately 43 kDa under reducing conditions; validate the observed molecular weight in your specific cell or tissue lysate.
• For ELISA applications, titrate the antibody against recombinant FAIM3 protein or cell lysates to determine optimal coating and detection concentrations.
• As a polyclonal antibody, lot-to-lot consistency may vary; include appropriate positive and negative controls and consider pre-adsorption if non-specific binding is observed.
• Cross-reactivity with mouse and rat FAIM3 is reported; however, sequence homology suggests potential binding to other species; validate in the relevant experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio bridges the gap between discovery and diagnostics, providing manufacturers, laboratories, and research institutes with one-stop access to premium IVD raw materials. The FAIM3 Rabbit pAb exemplifies our commitment to supplying validated polyclonal antibodies for demanding immunoassays; our portfolio also includes monoclonal antibodies, recombinant proteins, and ancillary reagents to support every stage of assay development. Whether you need rare-target antibody sourcing, bulk custom production, or technical consulting, CamelBio streamlines your pathway from concept to clinic.