Anti-FAH Rabbit Polyclonal Antibody for WB, ELISA - P16930

Core Product Specifications and Parameters

Biological Background: FAH Function and Localization

• Fumarylacetoacetase (FAH) is a hydrolase enzyme that catalyzes the final step in the tyrosine degradation pathway, converting fumarylacetoacetate into fumarate and acetoacetate.
• As a key enzyme in phenylalanine and tyrosine catabolism, FAH plays a critical role in the metabolism of aromatic amino acids and lipid-related intermediates.
• FAH is mainly expressed in the liver and kidney, with lower levels detected in many other tissues, reflecting its central role in hepatic amino acid degradation.
• The enzyme requires divalent metal ions such as calcium and magnesium for its catalytic activity.
• Alternative splicing of the FAH gene produces multiple transcript variants, potentially contributing to functional diversity.
• Post-translational modifications include acetylation and phosphorylation, which may regulate enzyme activity or stability.
• Mutations in the FAH gene are associated with disease variants, leading to hereditary tyrosinemia type I, a severe metabolic disorder characterized by liver and kidney dysfunction.

Experimental Guidance and Technical Tips

• The immunogen used is a full-length recombinant human FAH protein (amino acids 1-419). This polyclonal antibody may detect multiple epitopes, potentially providing robust signals in applications such as Western blot and ELISA.
• For Western blot, the predicted molecular weight of FAH is approximately 46 kDa. Ensure complete denaturation and reduction of samples, and validate the antibody’s reactivity in the relevant species and tissue types (e.g., liver or kidney lysates).
• For ELISA, the antibody can be used for capture or detection depending on the assay format. Consider pairing with a matched monoclonal antibody for sandwich ELISA development, and optimize coating and blocking conditions.
• Cross-reactivity has been observed with human, mouse, and rat FAH; however, users should verify performance in their specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to providing diagnostic manufacturers, laboratories, and research institutes with a comprehensive range of IVD raw materials and technical support. This FAH polyclonal antibody is part of our extensive portfolio targeting metabolic disease markers, and we also offer validated antibody pairs, monoclonal antibodies, and bulk ancillary reagents to streamline your assay development. For rare or custom targets, CamelBio excels in sourcing and supply chain management, helping you bridge from concept to clinical application efficiently.