Anti-FAH Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P16930

Core Product Specifications and Parameters

Biological Background: FAH Function and Localization

• Fumarylacetoacetase (FAH), also known as beta-diketonase or fumarylacetoacetate hydrolase, is an enzyme encoded by the FAH gene.
• It is predicted to function as a hydrolase involved in the catabolism of phenylalanine and tyrosine, playing a role in lipid metabolism.
• According to keyword annotations, FAH requires calcium or magnesium as metal cofactors (metal-binding) and undergoes post-translational modifications including acetylation and phosphorylation.
• Alternative splicing of the FAH gene produces multiple transcript variants, and mutations are linked to disease variants (Keyword: Disease variant).
• The protein is mainly expressed in liver and kidney, with lower levels detected in many other tissues (Tissue specificity).

Experimental Guidance and Technical Tips

• The immunogen comprises the full-length human FAH protein (amino acids 1-419), suggesting the antibody can recognize both native and denatured conformations, supporting applications such as WB, IHC-P, IF/ICC, and ELISA.
• For WB, expect a band at approximately 46 kDa (Protein Weight). Adjust loading controls and antibody dilutions accordingly.
• In IHC-P and IF/ICC, validation with positive control tissues (e.g., human liver or kidney) is recommended; background may be minimized by optimizing antigen retrieval and blocking conditions.
• Due to cross-reactivity with mouse and rat, this antibody is suitable for studies in rodent models; always include species-matched controls.
• As with any antibody, empirical determination of optimal working concentrations and incubation conditions is critical for reproducible results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated one-stop sourcing partner for IVD raw materials, offering access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and support for rare target sourcing. The FAH antibody is relevant for research into tyrosine/phenylalanine catabolism, metabolic disorders, and liver/kidney disease, fitting within our portfolio of high-quality reagents for diagnostic development. Reach out to explore how CamelBio can streamline your supply chain from concept to clinic.