Anti-FAH Polyclonal Antibody for WB, IF/ICC, ELISA - P16930

Core Product Specifications and Parameters

Biological Background: FAH Function and Localization

• Fumarylacetoacetase (FAH), also known as beta-diketonase or fumarylacetoacetate hydrolase, is encoded by the FAH gene in humans. Alternative splicing generates multiple isoforms.
• As indicated by its annotation, FAH functions as a hydrolase in the final step of phenylalanine and tyrosine catabolism, catalyzing the hydrolysis of fumarylacetoacetate to fumarate and acetoacetate.
• The enzyme requires divalent metal ions, specifically magnesium and calcium, for its catalytic activity.
• Post-translational modifications of FAH include acetylation and phosphorylation, identified through direct protein sequencing and proteomics analyses.
• FAH is predominantly expressed in liver and kidney, with lower detection in various other tissues.
• Disease-associated variants of FAH are linked to metabolic disorders, particularly tyrosinemia type I.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human FAH, potentially allowing recognition of multiple isoforms; verify specificity in your experimental system.
• For Western blot, the expected protein weight is ~46 kDa; validate the detected band using appropriate positive controls (e.g., liver or kidney lysates) and consider blocking conditions to reduce non-specific binding.
• In immunofluorescence (IF/ICC), test various fixation and permeabilization methods to achieve optimal nuclear/cytoplasmic staining based on the expected subcellular distribution of FAH.
• For ELISA, the antibody pair compatibility should be determined empirically; optimize coating concentration and detection conditions for best signal-to-noise ratio.
• Since the antibody shows cross-reactivity with mouse and rat, it may be suitable for comparative studies across species; confirm reactivity in the relevant model organism.
• Always include appropriate positive and negative controls to ensure assay performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research laboratories, offering this anti-FAH polyclonal antibody alongside validated antibody pairs, bulk ancillary reagents, and custom raw-material solutions. With a focus on metabolic disorder research areas, we help streamline your procurement from discovery to clinical application.