Anti-FAF2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q96CS3

Core Product Specifications and Parameters

Biological Background: FAF2 Function and Localization

• FAF2 (FAS-associated factor 2), also known as UBXD8, is a UBX domain-containing protein encoded by the FAF2 gene in humans.
• It functions in endoplasmic reticulum-associated degradation (ERAD), facilitating ubiquitin-dependent degradation of misfolded ER proteins. Related references: PMID:18711132, PMID:24215460
• FAF2 regulates insulin-like growth factor receptor signaling by controlling IGF1R steady-state expression. Related references: PMID:26692333
• At lipid droplets, FAF2 recruits the ATPase VCP and binds to PNPLA2/ATGL, inhibiting triacylglycerol hydrolysis and reducing lipid droplet degradation. Related references: PMID:23297223
• It participates in stress granule disassembly by associating with ubiquitinated G3BP1, promoting VCP-mediated extraction from stress granules. Related references: PMID:34739333
• FAF2 localizes to the cytoplasm, lipid droplets, and endoplasmic reticulum.
• Broadly expressed with highest levels in brain.
• Post-translational modifications include acetylation; the protein contains a coiled coil domain and is involved in the unfolded protein response.

Experimental Guidance and Technical Tips

• The immunogen covers the middle portion of FAF2 (aa 176-393); consider using this sequence for peptide blocking or epitope mapping studies.
• For Western blot, a band around 53 kDa (endogenous FAF2) may be detected; confirm specificity using positive controls (e.g., HeLa, HepG2) and negative controls (knockdown/knockout).
• In immunofluorescence/ICC, fix and permeabilize cells appropriately; co-staining with organelle markers can verify localization to lipid droplets or ER.
• For ELISA, the antibody can be used as capture or detection reagent; optimize coating and detection conditions.
• Cross-reactivity with mouse and rat suggests conserved epitopes; verify in relevant models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers IVD and research communities with one-stop access to high-quality raw materials, including validated antibody pairs, optimized polyclonal antibodies like this FAF2 antibody, and bulk ancillary reagents. Our portfolio supports diverse applications from ER stress and lipid droplet biology to neurodegeneration research. Leverage our sourcing expertise for rare target raw materials and accelerate your assay development from concept to clinic.