Anti-FAF1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9UNN5

Core Product Specifications and Parameters

Biological Background: FAS-associated factor 1 Function and Localization

• FAF1 (FAS-associated factor 1), also known as UBXD12 and UBXN3A, is a ubiquitin-binding protein (PubMed:19722279). Related references: PMID:19722279

• It is required for the progression of DNA replication forks by targeting the DNA replication licensing factor CDT1 for degradation (PubMed:26842564). Related references: PMID:26842564

• Potentiates FAS-induced apoptosis but cannot initiate it independently (By similarity).

• Localizes predominantly to the nucleus.

• Tissue expression: most abundant in testis, slightly less in skeletal muscle and heart, then prostate, thymus, ovary, small intestine, and colon; undetectable in peripheral blood leukocytes.

• Classified as a phosphoprotein and undergoes alternative splicing; also designated reference proteome and 3D-structure.

• Predicted molecular weight is 73,954 Da, migrating at approximately 74 kDa on western blots.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide; this antibody likely detects linear epitopes, making it suitable for denaturing WB conditions. Validate in your sample system.

• For IHC-P, consider using testis or skeletal muscle sections as positive controls due to high FAF1 expression; optimize antigen retrieval and antibody concentration.

• Cross-reactivity observed with mouse and rat; confirm sequence homology if using beyond human, and validate in the relevant species.

• In ELISA, the peptide-based design suggests reactivity with recombinant or native FAF1; include negative controls (peptide blocking) to confirm specificity.

• Expected band size: ~74 kDa; use molecular weight markers and a positive control lysate (e.g., HeLa, testis) for WB confirmation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop platform providing diagnostic manufacturers, laboratories, and research institutes with IVD raw materials, technical services, and consulting from concept to clinic. This Anti-FAF1 polyclonal antibody supports investigations into apoptosis, ubiquitin-mediated proteolysis, and DNA replication. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare-target raw materials—ensuring your project’s continuity.