Anti-FADS2 Polyclonal Antibody for WB and ELISA - O95864

Core Product Specifications and Parameters

Biological Background: Acyl-CoA 6-desaturase (FADS2) Function and Localization

• FADS2 encodes acyl-CoA 6-desaturase, also known as delta(6) fatty acid desaturase (D6D) or fatty acid desaturase 2, a key enzyme in highly unsaturated fatty acid (HUFA) biosynthesis.
• It catalyzes the rate-limiting first step: introduction of a cis double bond at carbon 6 of linoleic acid (LA, 18:2n-6) and alpha-linolenic acid (ALA, 18:3n-3) to produce gamma-linoleate (GLA) and stearidonate, respectively. Related references: PMID:12713571
• In the n-3 HUFA pathway, it desaturates tetracosapentaenoate (24:5n-3) to tetracosahexaenoate (24:6n-3), a precursor for docosahexaenoate (DHA), crucial for nervous system function (by similarity).
• FADS2 also converts palmitate to sapienate, a unique human sebum fatty acid with roles in acne development and antimicrobial defense. Related references: PMID:12713571
• It exhibits secondary delta-8 desaturase activity with preference for n-3 fatty acyl-CoA substrates, and can desaturate trans-vaccenoate, the major trans fatty acid in human milk (by similarity).
• The enzyme is localized to the endoplasmic reticulum membrane and belongs to the fatty acid desaturase family, featuring transmembrane domains and oxidoreductase activity.
• Expression is ubiquitous but highest in liver, brain, lung, heart, and retina; reduced expression in breast tumors correlates with poor prognosis.
• The FADS2 gene undergoes alternative splicing, and its product is involved in electron transport and lipid metabolism.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1-100 of human FADS2 (NP_004256.1); this N-terminal region may detect multiple isoforms if conserved across splice variants.
• For Western blotting, the predicted molecular weight is 52 kDa, but consider possible shifts due to glycosylation or other modifications; validate in endogenous and overexpressing systems.
• For ELISA, pair with a matched detection antibody or use as a capture reagent; optimize coating concentration and blocking conditions for your specific plate format.
• As a rabbit polyclonal antibody, expect some lot-to-lot variation; titrate each new lot and include appropriate controls (e.g., knockout lysates or peptide competition) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted partner for diagnostic manufacturers and research laboratories, providing end-to-end access to validated IVD raw materials, from concept to clinic. This anti-FADS2 polyclonal antibody supports fatty acid metabolism and dermatology research, and we can supply complementary reagents such as recombinant proteins, antibody pairs, and optimized monoclonal antibodies for assay development. Our sourcing capabilities extend to rare targets and bulk ancillary reagents, ensuring continuity and scalability for your projects.