Anti-FADS1 Rabbit Polyclonal Antibody for WB, ELISA - O60427

Core Product Specifications and Parameters

Biological Background: FADS1 Function and Localization

• FADS1, also known as delta-5 fatty acid desaturase (D5D), is an endoplasmic reticulum and mitochondrial membrane protein that introduces a cis double bond at carbon 5 of fatty acyl-CoA substrates.
• It catalyzes the rate-limiting step in the biosynthesis of highly unsaturated fatty acids (HUFAs), converting dihomo-gamma-linoleoate (DGLA; 20:3n-6) to arachidonic acid (AA; 20:4n-6) and eicosatetraenoate (ETA; 20:4n-3) to eicosapentaenoic acid (EPA; 20:5n-3).
  Related references:
  PMID:10601301 PMID:10769175
• As a key regulator of inflammatory lipid metabolism, FADS1 controls production of prostaglandin E2 and other eicosanoids, thereby influencing acute inflammatory responses and epithelial homeostasis.
• It contributes to membrane phospholipid composition by supplying arachidonic acid for incorporation into phospholipids, including phosphatidylinositol-4,5-bisphosphate, which modulates inflammatory cytokine production in T-cells.
• FADS1 also desaturates trans-vaccenoate (18:1n-9), a metabolite from the biohydrogenation of linoleic acid.
• Widely expressed, FADS1 shows highest levels in liver, brain, adrenal gland, and heart, with notable expression during fetal development.
• The protein undergoes acetylation and alternative splicing, and functions as an oxidoreductase in fatty acid metabolism.

Experimental Guidance and Technical Tips

• The immunogen (aa 100–200) is conserved across human FADS1; cross-reactivity studies in mouse and rat are recommended to confirm specificity by sequence alignment.
• For Western blot, a band at ~52 kDa is expected; however, cleavage, acetylation, or other modifications may shift the apparent molecular weight—validate in relevant tissue lysates (e.g., liver or brain).
• ELISA applications may require optimization of coating antigen and antibody titration for maximal signal-to-noise ratio.
• As a rabbit polyclonal antibody, lot-to-lot consistency in titer and affinity should be verified before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined procurement pathway for IVD raw materials, from monoclonal/polyclonal antibodies like this FADS1 rabbit pAb to bulk ancillary reagents. Our portfolio supports fatty acid and lipid metabolism research by providing validated antibody pairs, optimized detection reagents, and access to rare-target raw materials. With end-to-end sourcing solutions and technical consulting, we help diagnostic manufacturers accelerate development from concept to clinic.