Anti-FADS1 Monoclonal Antibody for WB and ELISA - O60427

Core Product Specifications and Parameters

Biological Background: FADS1 Function and Localization

• FADS1 (Fatty Acid Desaturase 1) is an acyl-CoA (8-3)-desaturase that introduces a cis double bond at carbon 5 of fatty acyl chains. It is also known as Delta(5) fatty acid desaturase (D5D).
• It plays a key role in the biosynthesis of highly unsaturated fatty acids (HUFAs) from essential polyunsaturated fatty acids (PUFAs) like linoleic acid and alpha-linolenic acid.
• Specifically, it desaturates dihomo-gamma-linoleate (DGLA, 20:3n-6) to arachidonate (AA, 20:4n-6) and eicosatetraenoate (ETA, 20:4n-3) to eicosapentaenoate (EPA, 20:5n-3), a rate-limiting step in eicosanoid production. Related references: PMID:10601301, PMID:10769175
• FADS1 is a membrane-bound enzyme localized primarily to the endoplasmic reticulum and also detected in mitochondria.
• It is widely expressed, with highest levels in liver, brain, adrenal gland, and heart, and is abundant in fetal liver and brain.
• The protein contains several transmembrane helices and is subject to post-translational modifications including acetylation and alternative splicing.
• FADS1 controls the metabolism of inflammatory lipids, such as prostaglandin E2, contributing to acute inflammatory response and epithelial homeostasis.
• It does not exhibit catalytic activity toward 20:3n-6 but may enhance FADS2 activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 50-150 of human FADS1; this region may be conserved across species, supporting cross-reactivity with mouse and rat.
• For Western blotting, the predicted molecular weight is ~52 kDa; consider using appropriate molecular weight markers and validate band size in relevant tissue or cell lysates.
• ELISA applications may require optimization of coating antigen and detection conditions; consider pairing with a matched antibody pair for sandwich ELISA.
• Validate antibody performance in the relevant sample system, as expression levels can vary across tissues and cell types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to IVD raw materials, from validated antibody pairs to bulk ancillary reagents. Our FADS1 rabbit monoclonal antibody supports studies in fatty acid metabolism and inflammatory pathways, and we offer customized sourcing for rare targets and application-optimized antibodies. Contact us to leverage our end-to-end supply chain and technical consulting for your next diagnostic development project.