Anti-FADD Rabbit Polyclonal Antibody for WB, IP, ELISA - Q13158

Core Product Specifications and Parameters

Biological Background: FADD Function and Localization

• FADD (FAS-associated death domain protein), also known as MORT1 or GIG3, is a 23 kDa adaptor protein belonging to the death domain superfamily, encoded by the FADD gene.
• FADD serves as an essential apoptotic adapter by recruiting caspase-8 (CASP8) or caspase-10 to activated death receptors, forming the death-inducing signaling complex (DISC) and subsequently triggering the caspase cascade leading to apoptosis. Related references: PMID:16762833, PMID:19118384, PMID:20935634
• Beyond apoptosis, FADD plays a role in interferon-mediated antiviral innate immune responses by positively regulating interferon signaling pathways. Related references: PMID:21109225, PMID:24204270
• Subcellularly, FADD is predominantly localized in the cytoplasm.
• FADD is widely expressed across various tissues, with the notable exception of peripheral blood mononuclear leukocytes.
• Key post-translational modifications of FADD include phosphorylation and glycosylation, which may regulate its function and interactions.
• FADD is implicated in disease states and host-virus interactions, highlighting its critical role in immune regulation and pathology.

Experimental Guidance and Technical Tips

• The immunogen spans nearly the full-length human FADD protein (aa 1-208); this polyclonal antibody may recognize multiple epitopes and could detect both full-length and cleaved fragments.
• For Western blotting, a band around 23 kDa is expected; consider validating detection in your sample system with appropriate loading controls.
• The antibody is listed for immunoprecipitation (IP) and ELISA; consider optimizing antibody dilution, incubation conditions, and buffer systems for these applications.
• Reported cross-reactivity with mouse and rat FADD; verify specificity and sensitivity in your experimental models if using these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic and research laboratories with streamlined access to high-quality IVD raw materials like this FADD antibody. Our portfolio includes validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents for apoptosis and immune signaling research. From concept to clinic, we support your assay development with reliable sourcing and technical consultation.