Anti-FADD Rabbit Monoclonal Antibody for WB, ELISA - Q13158

Core Product Specifications and Parameters

Biological Background: FADD Function and Localization

• FAS-associated death domain protein (FADD), also known as MORT1 and GIG3, is a critical adapter molecule in death receptor-mediated apoptosis and innate immune signaling.
• Acts as an apoptotic adaptor that recruits caspases CASP8 or CASP10 to activated FAS/CD95 or TNFRSF1A/TNFR-1 receptors, forming the death-inducing signaling complex (DISC) to trigger caspase activation and apoptosis. Related references: PMID:16762833, PMID:19118384, PMID:20935634
• Participates in interferon-mediated antiviral innate immune responses by positively regulating interferon signaling pathways. Related references: PMID:21109225, PMID:24204270
• Localizes predominantly to the cytoplasm, consistent with its role as a signal transduction mediator.
• Widely expressed across human tissues, with the notable exception of peripheral blood mononuclear leukocytes.
• Post-translational modifications include phosphorylation and glycosylation, which may regulate its activity and interactions.

Experimental Guidance and Technical Tips

• For Western blotting, use whole cell lysates from human cell lines (e.g., HeLa or Jurkat) under reducing conditions; a specific band near 23 kDa is expected. Validate with a FADD-positive control.
• In ELISA, the recombinant immunogen (aa 20-150) can serve as a positive coating antigen. Perform checkerboard titrations to optimize antibody and antigen concentrations.
• As FADD is primarily cytoplasmic, subcellular fractionation or immunofluorescence co-staining may improve detection specificity. Include appropriate isotype controls for comparison.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined one-stop supply chain for IVD raw materials, supporting your projects from concept to clinic. Our FADD Rabbit Monoclonal Antibody is an essential tool for apoptosis, cancer biology, and immunology research, complemented by our wide portfolio of validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents. Contact us to source rare targets or customize your raw material needs with dependable technical support.