Anti-FADD Polyclonal Antibody for WB, IP, IF-P, ELISA - Q61160

Core Product Specifications and Parameters

Biological Background: FADD Function and Localization

• FADD (FAS-associated death domain protein, also known as Mort1/FADD) is an essential adaptor molecule in extrinsic apoptosis signaling.
• It recruits initiator caspases CASP8 or CASP10 to activated death receptors (FAS/CD95 or TNFRSF1A/TNFR-1), forming the death-inducing signaling complex (DISC) and triggering the caspase cascade leading to apoptosis.
• Beyond apoptosis, FADD participates in interferon-mediated antiviral innate immune responses, positively regulating interferon signaling pathways.
• Subcellularly, FADD is predominantly localized in the cytoplasm.
• Post-translational modification by phosphorylation and its involvement in immunity are highlighted by UniProt keywords: Phosphoprotein, Apoptosis, Immunity, Innate immunity.
• The predicted molecular weight of mouse FADD is ~23 kDa (22,960 Da), consistent with the observed 23 kDa band.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-205 of mouse FADD, covering the death domain and death effector domain; this full-length recombinant protein suggests the antibody may recognize both full-length and possibly cleaved forms of FADD.
• For Western blot, a specific band around 23 kDa is anticipated. Consider using knockdown or knockout controls to confirm specificity, especially in samples where FADD expression is low.
• In immunoprecipitation, the antibody may be used to pull down the DISC complex; optimize the antibody concentration and lysis conditions to preserve protein interactions.
• For immunofluorescence/IF-P, FADD shuttles between cytoplasm and receptor complexes; permeabilization and fixation protocols should be validated to detect dynamic localization.
• ELISA applications can employ the recombinant immunogen as a standard for quantitative detection; cross-reactivity across human, mouse, and rat broadens utility in comparative studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw-material sourcing by providing validated antibodies, matched pairs, and ancillary reagents for apoptosis and innate immunity research. Our one-stop platform supports diagnostic manufacturers with rare-target antibody procurement and bulk supply, accelerating concept-to-clinic development. Partner with CamelBio to customize your sourcing needs for FADD-related assays and beyond.