Anti-FADD Monoclonal Antibody for WB, IHC-P, ELISA - Q61160

Core Product Specifications and Parameters

Biological Background: FADD Function and Localization

• FADD (FAS-associated death domain protein), also known as Mort1, is an apoptotic adapter molecule.
• It recruits CASP8 or CASP10 to activated FAS/CD95 or TNFRSF1A/TNFR-1 receptors, forming the death-inducing signaling complex (DISC).
• Within the DISC, CASP8 undergoes proteolytic activation, initiating the downstream caspase cascade that executes apoptosis.
• Beyond apoptosis, FADD is involved in interferon-mediated antiviral immune response, positively regulating interferon signaling.
• FADD localizes primarily to the cytoplasm.
• It is a phosphoprotein, suggesting regulation by phosphorylation.
• Structural studies have elucidated the death domain interactions critical for its function.

Experimental Guidance and Technical Tips

• For Western blot (WB), the expected molecular weight is approximately 23 kDa; validate specificity using relevant positive and negative controls (e.g., FADD knockout cell lysates).
• In immunohistochemistry (IHC-P), consider appropriate antigen retrieval methods and validate staining patterns in tissues known to express FADD, such as lymphoid organs.
• Since FADD is a cytoplasmic protein, ensure permeabilization steps are optimized for intracellular staining in IHC and immunofluorescence.
• This rabbit monoclonal antibody is also suitable for ELISA; it may be evaluated as a capture or detection reagent in sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a one-stop platform for sourcing high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Whether your project involves the FAS/TNF death receptor pathway, apoptosis, or innate immunity, our supply network can support your development from concept to clinic. Contact us to discuss customized sourcing solutions for rare targets like FADD.