Anti-FABPI Polyclonal Antibody for WB, ELISA - P12104

Core Product Specifications and Parameters

Biological Background: Fatty acid-binding protein, intestinal Function and Localization

• Encoded by the FABP2 gene, fatty acid-binding protein, intestinal (also known as I-FABP or FABPI) is a ~15 kDa cytosolic lipid-binding protein belonging to the intracellular fatty acid-binding protein family.
• Plays a key role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters, facilitating their movement within the cytoplasm.
• Involved in triglyceride-rich lipoprotein synthesis, contributing to dietary fat absorption and chylomicron assembly in enterocytes.
• Exhibits high binding affinity for saturated long-chain fatty acids and relatively lower affinity for unsaturated long-chain fatty acids.
• Functions as a lipid sensor, potentially helping to maintain energy homeostasis by modulating lipid-mediated signaling.
• Primarily expressed in the small intestine, with highest levels in the jejunum, and much lower expression in the large intestine; restricted to absorptive epithelial cells.
• Post-translationally modified by acetylation; also identified in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein comprising amino acids 1-132 of human FABPI, ensuring recognition of full-length endogenous protein. For immunoblotting, the predicted molecular weight is ~15 kDa; validate band size in relevant tissue lysates (e.g., jejunum or small intestine).
• This polyclonal antibody is suitable for WB and ELISA applications. Consider optimizing dilutions for your specific sample type and detection system. Endogenous expression in intestinal tissues and cross-reactivity with mouse and rat may facilitate translational studies.
• Since FABPI is a cytoplasmic protein, standard whole-cell or tissue lysates can be used. For localization studies, additional fixation or permeabilization steps may be required if extended to ICC/IHC; however, these applications are not validated, so validate accordingly.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated one-stop sourcing bridge for IVD manufacturers, CamelBio offers validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and custom procurement of rare targets. This anti-FABPI antibody supports research into intestinal fatty acid transport and metabolic disorders, complementing our portfolio of lipid metabolism and gastrointestinal disease raw materials. From concept to clinic, we streamline your raw material supply chain with technical consultation and reliable delivery.