Anti-FABP6 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P51161

Core Product Specifications and Parameters

Biological Background: FABP6 Function and Localization

• FABP6 (Fatty acid-binding protein 6), also known as gastrotropin, ileal lipid-binding protein (ILBP), or intestinal bile acid-binding protein (I-BABP), is a member of the intracellular fatty acid-binding protein family that specifically binds bile acids with high affinity.
• It plays a central role in enterohepatic bile acid metabolism by facilitating the apical-to-basolateral transport of conjugated bile acids in ileal enterocytes, with preference for deoxycholic > cholic > chenodeoxycholic acids and taurine-conjugated > glycine-conjugated > unconjugated forms.
• FABP6 is essential for the survival of colon cancer cells exposed to bile acid-induced apoptosis, highlighting its cytoprotective role in the gastrointestinal tract.
• The protein stimulates gastric acid and pepsinogen secretion, linking bile acid sensing to digestive enzyme release.
• Two isoforms exist due to alternative promoter usage: isoform 1 is expressed in the jejunum, ileum, cecum, and ascending colon; isoform 2 shows a broader distribution in the gallbladder, duodenum, and entire colon, and is detected in colorectal adenocarcinomas and adjacent normal mucosa.
• Subcellularly, FABP6 localizes to the cytoplasm and associates with membranes, consistent with its lipid transport function.
• Post-translational modification includes acetylation, as identified by proteomics, though its functional impact remains to be fully characterized.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–128 of human FABP6 (NP_001436.1), which covers nearly the entire protein; this full-length coverage may enable recognition of both isoforms and native conformations.
• For Western blot (WB), a band around 14 kDa is expected; consider using fresh ileal or colon tissue lysates with protease inhibitors, and validate signal specificity using knockdown or knockout controls if available.
• For immunofluorescence (IF-P), standard fixation/permeabilization protocols (e.g., 4% PFA with 0.1% Triton X-100) are suitable; note that FABP6 may exhibit both cytoplasmic and membrane-associated staining patterns.
• In ELISA applications, the polyclonal nature of the antibody can provide robust capture/detection pairing after appropriate titer optimization; confirm linearity and sensitivity using recombinant FABP6 protein.
• Given cross-reactivity with mouse and rat, this antibody is a useful tool for preclinical models of bile acid metabolism or colon cancer; however, always verify reactivity in your specific tissue and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in connecting diagnostic manufacturers and research teams with premium IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents. For gastrointestinal and bile acid research, we offer a reliable supply of FABP6-targeting antibodies and can source additional rare targets from our global network. Let CamelBio streamline your procurement process from concept to clinic.