Anti-FABP5 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q01469

Core Product Specifications and Parameters

Biological Background: FABP5 Function and Localization

• FABP5 is an intracellular carrier for long-chain fatty acids and related active lipids, such as endocannabinoids, regulating their metabolism and signaling.
• Selectively delivers specific fatty acids from the cytosol to the nucleus, activating nuclear receptors including PPARδ, thereby promoting proliferation and survival. Related references: PMID:21395585 PMID:22170058
• Functions as a synaptic carrier of endocannabinoids at central synapses, controlling retrograde signaling.
• Modulates inflammation by regulating PTGES induction via NF-κB activation and PGE2 biosynthesis.
• Highly expressed in keratinocytes, especially in psoriatic skin, suggesting a role in keratinocyte differentiation. Related references: PMID:8092987
• Localizes to the cytoplasm, nucleus, synapse, postsynaptic density, and is also secreted.
• Undergoes post-translational modifications including acetylation, phosphorylation, and contains disulfide bonds.
• Belongs to the fatty acid-binding protein family and is also known as epidermal-type fatty acid-binding protein (E-FABP).

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 1-135 of human FABP5 (full-length), which may contribute to detecting the endogenous protein.
• Based on the immunogen sequence and cross-reactivity data (Human, Mouse, Rat), the antibody likely recognizes FABP5 across these species; validation in each species is advised.
• For WB, consider using a blocking agent compatible with rabbit polyclonal antibodies and validate the expected band at ~15 kDa.
• For IHC-P, tissue fixation and antigen retrieval conditions should be optimized for each sample type.
• For IF/ICC, appropriate fixation and permeabilization methods should be tested.
• ELISA performance can be evaluated with recombinant FABP5 protein or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and laboratories with one-stop access to IVD raw materials, technical services, and consulting. This anti-FABP5 polyclonal antibody supports your research into fatty acid transport, inflammation, and keratinocyte biology. Explore our comprehensive portfolio, including validated antibody pairs and bulk ancillary reagents, to accelerate your assay development from concept to clinic.