Anti-FABP5 Rabbit Monoclonal Antibody for WB, IHC, IF, ELISA - Q01469

Core Product Specifications and Parameters

Biological Background: FABP5 Function and Localization

• FABP5, also known as epidermal-type fatty acid-binding protein (E-FABP) or psoriasis-associated fatty acid-binding protein homolog (PA-FABP), is a member of the fatty acid-binding protein family. It functions as an intracellular carrier for long-chain fatty acids and related active lipids, such as endocannabinoids, regulating the metabolism and actions of bound ligands.
• In addition to cytosolic transport, FABP5 selectively delivers specific fatty acids, including retinoic acid, from the cytosol to the nucleus, where they activate nuclear receptors like peroxisome proliferator-activated receptor delta (PPARδ), thereby influencing cell proliferation and survival.
  Related references: PMID:21395585, PMID:22170058
• By similarity, FABP5 modulates inflammation by regulating prostaglandin E2 (PGE2) biosynthesis via NF-kappa-B activation, suggesting a role in inflammatory responses.
• May be involved in keratinocyte differentiation, consistent with its high expression in psoriatic skin.
  Related references: PMID:8092987
• FABP5 is localized in the cytoplasm, nucleus, and at synapses (postsynaptic density); it is also secreted. In brain gray matter, it may serve as a synaptic carrier of endocannabinoids, controlling retrograde signaling.
  Related references: PMID:21395585
• Post-translational modifications include acetylation, phosphorylation, and formation of disulfide bonds, which may regulate its lipid-binding and transport activities.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1–135), which is highly conserved across human, mouse, and rat, supporting the listed cross-reactivity. For novel sample types, validate cross-reactivity by sequence alignment or preliminary testing.
• For Western blot, a band near 15 kDa is expected; use lysates from FABP5-expressing tissues such as skin or brain as positive controls.
• For immunofluorescence (IF) and immunohistochemistry (IHC), consider using skin (preferably psoriatic) or brain sections; optimize fixation and permeabilization to detect secreted and intracellular pools.
• For ELISA applications, confirm appropriate coating buffers and detection antibodies; consider pairing with a matched antibody to develop a sandwich ELISA for quantitative detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with integrated access to IVD raw materials, streamlining the journey from concept to clinic. Our FABP5 antibody supports studies in lipid metabolism, inflammation, and dermatology, and we further offer validated antibody pairs, bulk ancillary reagents, and custom monoclonal antibody production to meet your project's scale and specificity needs. For rare or emerging targets, our global sourcing network can secure compliant raw materials quickly.