Anti-FABP4 Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P15090

Core Product Specifications and Parameters

Biological Background: FABP4 Function and Localization

• FABP4 (Fatty acid-binding protein, adipocyte), also known as aP2, ALBP, A-FABP, or AFABP, belongs to the intracellular fatty acid-binding protein family.
• It functions as a lipid transport protein in adipocytes, binding both long-chain fatty acids and retinoic acid, and shuttling these ligands to their cognate nuclear receptors.
• Subcellularly, FABP4 localizes to both the cytoplasm and the nucleus, consistent with its role in delivering lipophilic ligands to nuclear targets.
• Post-translational modifications such as phosphorylation and acetylation may regulate its lipid-binding activity and intracellular trafficking.
• As an adipocyte-specific marker, FABP4 is predominantly expressed in adipose tissue and is widely used as a readout for adipocyte differentiation and metabolic function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human FABP4 (residues 1–132), which may support robust recognition of the native protein across multiple applications.
• For Western blot (WB), a band of approximately 15 kDa is expected; consider using low‑molecular‑weight markers and optimizing transfer conditions for small proteins.
• In immunohistochemistry (IHC‑P) and immunofluorescence (IF‑P), antigen retrieval and permeabilization steps should be tailored to adipocyte‑rich tissue samples.
• Cross‑reactivity with mouse and rat FABP4 is reported, facilitating translational studies, but validation in the relevant species and sample system is recommended.
• For ELISA, the antibody can be explored as either a capture or detection reagent after appropriate pairing validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, providing reliable access to validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. This anti‑FABP4 polyclonal antibody supports research in adipocyte biology and metabolic disorders, and our team can assist with sourcing rare targets and fulfilling custom reagent needs to accelerate your diagnostic development from concept to clinic.