Anti-FABP4 Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P15090

Core Product Specifications and Parameters

Biological Background: FABP4 Function and Localization

• FABP4 (fatty acid-binding protein 4), also known as adipocyte fatty acid-binding protein (A-FABP), ALBP, or aP2, is a cytoplasm–nucleus shuttling lipid chaperone predominantly expressed in adipocytes.
• It binds long-chain fatty acids and retinoic acid with high affinity, and delivers these hydrophobic ligands to cognate nuclear receptors, thereby modulating gene transcription.
• FABP4 belongs to the intracellular fatty acid-binding protein (FABP) family, characterized by a conserved β-barrel structure that encloses the lipid ligand.
• Localized to both the cytoplasm and nucleus, FABP4 facilitates the subcellular trafficking of lipids and retinoids.
• Post-translational modifications such as acetylation and phosphorylation have been identified on FABP4; these may regulate its ligand-binding properties or compartment-specific functions.
• UniProt keywords (Lipid-binding, Transport, Cytoplasm, Nucleus, Phosphoprotein) underscore its role in intracellular lipid transport and signaling.
• The protein has a molecular weight of approximately 15 kDa (calculated 14.7 kDa from amino acid sequence), consistent with other FABP family members.

Experimental Guidance and Technical Tips

• The immunogen was a recombinant fusion protein encompassing amino acids 2–132 of human FABP4, representing the majority of the protein; this near-full-length design may favor recognition of the native conformation in assays like Western blot and immunohistochemistry.
• For Western blot, the small protein size (15 kDa) suggests running samples on a 10–15% SDS-PAGE gel and using appropriate low-molecular-weight markers for accurate verification.
• Reported cross-reactivity with mouse and rat FABP4 should be confirmed in the target tissue or cell line before using this antibody in those species; sequence homology supports this, but experimental validation is advised.
• In immunofluorescence (IF-P) and immunohistochemistry (IHC-P), optimize fixation and permeabilization to enable detection of both cytoplasmic and nuclear pools of FABP4.
• For ELISA applications, consider pairing this monoclonal antibody with a suitable detection antibody to build a sandwich assay, and test against recombinant FABP4 standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated sourcing partner for IVD raw materials, serving diagnostic manufacturers and research laboratories with a comprehensive portfolio from concept to clinic. For lipid metabolism targets like FABP4, we supply high-quality monoclonal antibodies, validated antibody pairs, and bulk ancillary reagents, alongside access to rare-target raw materials. Our end-to-end supply capabilities streamline assay development, allowing you to focus on innovation while we bridge the sourcing gap.