Anti-FABP12 Polyclonal Antibody for WB, ELISA - A6NFH5

Core Product Specifications and Parameters

Biological Background: FABP12 Function and Localization

• FABP12 (Fatty acid-binding protein 12) belongs to the intracellular lipid-binding protein family, characterized by a conserved β-barrel structure that facilitates hydrophobic ligand binding.
• Its primary function is suggested to be lipid transport, potentially shuttling fatty acids and other lipophilic molecules within the cell.
• The protein is notably expressed in retinoblastoma cell lines, indicating a possible role in cancer cell metabolism or signaling pathways.
• UniProt annotation includes keywords such as "Lipid-binding" and "Transport," and it is curated as a reference proteome member identified through large-scale proteomics.
• The theoretical molecular weight of FABP12 is approximately 15.6 kDa, consistent with the observed band near 16 kDa in applications.

Experimental Guidance and Technical Tips

• The immunogen spans nearly the entire FABP12 sequence (aa 1–140), so the polyclonal antibody likely recognizes multiple epitopes; this can improve sensitivity in denaturing Western blot conditions.
• For WB, consider using whole cell lysates from human or rat retinoblastoma cell lines as positive controls based on reported tissue specificity; validate in your specific sample system.
• In ELISA, the polyclonal nature may provide robust capture and detection; preliminary titration with recombinant FABP12 protein as standard is advisable.
• Confirm band identity by running molecular weight markers near 16 kDa and, if possible, use a blocking peptide competition to verify specificity.
• Cross-reactivity is documented for human and rat; if using mouse samples, additional validation is recommended as no data are provided.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes seamless access to a comprehensive portfolio of IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This Anti-FABP12 polyclonal antibody supports lipid metabolism and oncology research, and we can assist with sourcing validated antibody pairs, optimized monoclonal/polyclonal preparations, bulk ancillary reagents, and rare target raw materials to accelerate your development pipeline.