Anti-FABP1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P07148

Core Product Specifications and Parameters

Biological Background: FABP1 Function and Localization

• FABP1, also known as liver-type fatty acid-binding protein (L-FABP) or FABPL, is a cytosolic protein that binds free fatty acids, coenzyme A derivatives, bilirubin, and other small hydrophobic molecules, playing a role in intracellular lipid transport.
• It specifically participates in lipoprotein-mediated cholesterol uptake in hepatocytes and directly binds cholesterol, as shown in functional studies. Related references:
  • PMID:25732850
• FABP1 localizes to the cytoplasm, consistent with its role as an intracellular carrier for lipophilic ligands.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its lipid-binding activity and function.
• The protein is part of the reference proteome and has been widely studied by proteomics, with its 3D structure determined, highlighting its importance in lipid metabolism.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length protein (residues 1-127), so the antibody is expected to recognize native FABP1 in WB, IF/ICC, and ELISA.
• For WB, consider using a 12–15% SDS-PAGE gel to resolve the ~14 kDa FABP1 band.
• For IF/ICC, ensure adequate permeabilization to access the cytosolic FABP1 target.
• Validate cross-reactivity in mouse and rat samples if needed, as indicated by the supplier.
• Always optimize antibody dilution and assay conditions for the specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material sourcing partner, CamelBio supports research and diagnostic development in lipid metabolism and liver disease by offering validated antibodies like this FABP1 polyclonal antibody. From optimized monoclonal/polyclonal antibodies and antibody pairs to bulk ancillary reagents, CamelBio streamlines procurement for projects requiring rare or specialized targets.