Anti-FABP1 Rabbit Monoclonal Antibody for WB - P07148

Core Product Specifications and Parameters

Biological Background: FABP1 Function and Localization

• FABP1, also known as liver-type fatty acid-binding protein (L-FABP) or FABPL, is a member of the fatty acid-binding protein family that plays a critical role in intracellular lipid transport.
• It binds free fatty acids, their coenzyme A derivatives, bilirubin, cholesterol, and other small hydrophobic molecules in the cytoplasm.
• FABP1 facilitates lipoprotein-mediated cholesterol uptake in hepatocytes, contributing to hepatic lipid metabolism. Related references: PMID:25732850
• Subcellularly, it localizes to the cytoplasm where it performs its lipid chaperone functions.
• Post-translational modifications include acetylation and phosphorylation, as indicated by proteomics data, which may regulate its activity.
• Keywords such as 'Lipid-binding', 'Transport', and 'Acetylation' further highlight its role in metabolic processes.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide spanning amino acids 1-100 of human FABP1, a region that is highly conserved across human, mouse, and rat, consistent with the observed cross-reactivity.
• For Western blotting (WB), the expected protein band is approximately 14 kDa; use appropriate gel percentage and transfer conditions to resolve this small protein.
• In immunofluorescence (IF/ICC), optimize fixation and permeabilization to preserve cytoplasmic localization, and consider using appropriate controls.
• For ELISA applications, validate the antibody pair or detection system under standard conditions, taking into account potential nonspecific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers studying lipid metabolism and liver function, this FABP1 antibody is part of a broader supply chain that can support assay development from concept to clinic. Let CamelBio be your reliable partner in sourcing rare and critical raw materials for diagnostic applications.