Anti-FABP1 Polyclonal Antibody for WB, IF/ICC, ELISA - P07148

Core Product Specifications and Parameters

Biological Background: FABP1 Function and Localization

• The FABP1 gene encodes liver fatty acid-binding protein (L-FABP), also known as fatty acid-binding protein 1 (FABP1) or FABPL, a member of the intracellular lipid-binding protein family.
• FABP1 plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes by binding cholesterol. Related references: PMID:25732850
• It binds free fatty acids and their coenzyme A derivatives, bilirubin, and other small hydrophobic molecules in the cytoplasm, facilitating intracellular transport.
• Subcellular localization is primarily cytoplasmic, consistent with its role in intracellular lipid trafficking.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its function.
• Keywords: 3D-structure, Acetylation, Cytoplasm, Lipid-binding, Phosphoprotein, Proteomics identification, Reference proteome, Transport.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminus (aa 1–100) of human FABP1, suggesting the antibody may recognize denatured FABP1 and is suitable for WB. Consider optimizing denaturing conditions and loading controls.
• For IF/ICC, validate fixation and permeabilization protocols in your target cell line, as cytoplasmic antigens may require optimized permeabilization.
• In ELISA, a direct or sandwich format can be considered; pair this antibody with an appropriate detection antibody and validate in the relevant sample matrix.
• Ensure antibody specificity by including appropriate negative controls, especially when testing in species with high sequence homology (mouse, rat).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to supply validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents for lipid metabolism and liver function studies. Whether you need this anti-FABP1 antibody for assay development or are sourcing rare-target raw materials, we offer comprehensive support from concept to clinic. Contact us to streamline your IVD raw-material procurement with a trusted B2B partner.