Anti-FAAH2 Polyclonal Antibody for WB, IF/ICC, ELISA - Q6GMR7

Core Product Specifications and Parameters

Biological Background: FAAH2 Function and Localization

• Fatty-acid amide hydrolase 2 (FAAH2), also known as amidase domain-containing protein, anandamide amidohydrolase 2, or oleamide hydrolase 2, is encoded by the FAAH2 (AMDD) gene in humans.
• Catalyzes the hydrolysis of endogenous amidated lipids like the sleep-inducing lipid oleamide and the endocannabinoid anandamide, thereby regulating their signaling functions. Hydrolyzes monounsaturated anandamide preferentially over polyunsaturated substrates. Related references: PMID:17015445 PMID:19926788
• Subcellular localization includes membrane and lipid droplet, consistent with its role in lipid metabolism.
• Tissue expression is detected in kidney, liver, lung, prostate, heart, and ovary.
• Molecular weight is approximately 58 kDa; UniProt annotation lists a calculated mass of 58.3 kDa.
• Keywords: Hydrolase, Lipid degradation, Lipid droplet, Lipid metabolism, Transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 323-532 of human FAAH2, a region that may aid in detecting full-length protein in samples where the enzyme is expressed.
• For western blotting, consider using whole cell lysates from tissues known to express FAAH2, such as kidney or liver, and validate the observed band size around 58 kDa.
• Immunofluorescence/immunocytochemistry may require optimization of fixation and permeabilization protocols due to the membrane and lipid droplet localization of FAAH2.
• ELISA experiments can be designed using purified or recombinant protein as a standard; however, confirm reactivity in your matrix of interest.
• Cross-reactivity with mouse and rat is reported; verify that the antibody performs comparably in these species under your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials. This FAAH2 polyclonal antibody serves the lipid metabolism and endocannabinoid signaling research community. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your workflow from concept to clinic.