Anti-FAAH1 Rabbit Polyclonal Antibody for WB, ELISA - O00519

Core Product Specifications and Parameters

Biological Background: FAAH1 Function and Localization

• Fatty-acid amide hydrolase 1 (FAAH1), also known as FAAH-1 or PSAB, is an enzyme responsible for the degradation of bioactive fatty acid amides.
• Catalyzes the hydrolysis of endogenous amidated lipids, including the sleep-inducing lipid oleamide and the endocannabinoid anandamide, to their corresponding fatty acids, thereby regulating their signaling functions.
  Related references: PMID:17015445 PMID:19926788 PMID:9122178
• Preferentially hydrolyzes the polyunsaturated substrate anandamide over monounsaturated substrates.
  Related references: PMID:17015445 PMID:9122178
• Can also catalyze the hydrolysis of the endocannabinoid 2-arachidonoylglycerol.
  Related references: PMID:21049984
• Cooperates with PM20D1 to hydrolyze N-fatty acyl amino acids, acting as a physiological regulator of specific subsets of intracellular N-fatty acyl amino acids.
• Subcellularly localized to the endomembrane system, cytoplasm, and cytoskeleton.
• Highly expressed in the brain, small intestine, pancreas, skeletal muscle, and testis; also detected in kidney, liver, lung, placenta, and prostate.
• Contains a transmembrane domain and is subject to phosphorylation, classifying it as a phosphoprotein involved in lipid degradation.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to amino acids 450-579 of human FAAH1. Researchers may consider using this antibody to detect the C-terminal region of FAAH1.
• For Western blot applications, a band at approximately 63 kDa is expected. Validate the antibody in a relevant sample system, such as human or mouse brain lysate, where FAAH1 is highly expressed.
• When performing ELISA, consider using recombinant FAAH1 protein as a positive control to assess binding specificity.
• As this is a polyclonal antibody, potential batch-to-batch variability should be taken into account. It is recommended to optimize experimental conditions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutions with streamlined access to IVD raw materials, supporting the full development journey from concept to clinic. This FAAH1 polyclonal antibody is part of our extensive portfolio for endocannabinoid system research, and we can also assist with sourcing validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw materials to meet your specific project needs.