Anti-FA2H Rabbit Polyclonal Antibody for WB, ELISA - Q7L5A8

Core Product Specifications and Parameters

Biological Background: FA2H Function and Localization

• FA2H, also known as fatty acid alpha-hydroxylase or FAAH, is encoded by the FA2H gene in humans.
• Catalyzes the C-2 hydroxylation of free fatty acids to produce 2-hydroxy fatty acids, essential precursors for sphingolipids and glycosphingolipids in neural tissue and epidermis. Related references: PMID:15337768, PMID:15863841, PMID:17355976
• Exhibits stereospecificity for (R)-2-hydroxy fatty acids. Related references: PMID:22517924
• Plays a critical role in myelin sheath formation by contributing to galactosphingolipid synthesis.
• Essential for epidermal lamellar body formation and skin permeability barrier function. Related references: PMID:17355976
• Localizes to the endoplasmic reticulum and microsome membranes.
• Predominantly expressed in brain and colon, with lower levels in testis, prostate, pancreas, and kidney; also detected in differentiating keratinocytes and epidermis.
• Contains heme and iron binding sites, belongs to the fatty acid hydroxylase family, and is associated with hereditary spastic paraplegia and leukodystrophy.

Experimental Guidance and Technical Tips

• The immunogen (aa 95–170) targets a conserved region of FA2H; verify cross-reactivity with mouse and rat orthologs in relevant tissue samples.
• For Western blot, expect a band near 43 kDa. Use reducing conditions and include positive controls such as brain or colon lysates.
• In ELISA applications, titrate the antibody against recombinant FA2H or the immunogen peptide to optimize detection sensitivity.
• As FA2H is a membrane protein, employ lysis buffers containing detergents (e.g., Triton X-100 or SDS) for efficient protein extraction.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw-material sourcing, offering this anti-FA2H antibody as part of a comprehensive portfolio for lipid metabolism and neurological disease research. We supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and can source rare targets to support your diagnostic development from concept to clinic.