Anti-F4/80 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q61549

Core Product Specifications and Parameters

Biological Background: F4/80 Function and Localization

• F4/80, also known as EMR1 or Adhesion G protein-coupled receptor E1, is a cell surface glycoprotein belonging to the adhesion GPCR family.
• It contains EGF-like domains and a GPCR proteolytic site, suggesting a role in cell adhesion and transmembrane signaling.
• The protein is primarily expressed on the surface of macrophages, with low expression on blood monocytes, and is absent from macrophages in T-cell areas of lymph nodes and spleen.
• F4/80 functions as an orphan receptor involved in cell-cell interactions within the immune system, potentially contributing to regulatory T-cell development.
• Structural features include multiple transmembrane helices, disulfide bonds, and N-linked glycosylation, which are critical for its membrane localization and function.
• Keywords such as "Adaptive immunity" and "Glycoprotein" underscore its involvement in immune regulation and post-translational modification.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 367–645 within the extracellular domain of mouse F4/80; the antibody may favor detection of the glycosylated native protein in applications such as WB, IF/ICC, and ELISA.
• For Western blot, the predicted molecular weight is ~102 kDa; consider denaturing and reducing conditions optimized for membrane proteins.
• In immunofluorescence/ICC, fixation and permeabilization protocols should be carefully optimized to preserve membrane staining.
• Cross-reactivity with human F4/80 is reported; validate expression levels in your human cell or tissue system before interpretation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive sourcing solutions for IVD raw materials, including validated monoclonal and polyclonal antibodies, recombinant proteins, and bulk ancillary reagents. For investigators studying macrophage biology and immune regulation, CamelBio can streamline procurement of F4/80 antibodies and related targets, ensuring reliable supply for diagnostic development and research applications.