Anti-F4/80 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q61549

Core Product Specifications and Parameters

Biological Background: Adhesion G protein-coupled receptor E1 Function and Localization

• F4/80, also known as Adhesion G protein-coupled receptor E1 (ADGRE1) or EMR1, is a cell surface glycoprotein belonging to the adhesion GPCR family; it contains multiple EGF-like domains and a mucin-like stalk.
• The receptor functions as an orphan receptor involved in cell adhesion and cell-cell interactions specifically within the immune system; it may play a role in regulatory T-cell (Treg) development.
• ADGRE1 is characterized by a large extracellular region with EGF-like repeats, a GPS domain, and a seven-transmembrane helix; these features support both adhesive and signaling functions.
• Subcellularly, F4/80 is localized predominantly at the cell membrane, consistent with its role as a surface receptor.
• Tissue specificity studies in mouse show high expression on tissue-resident macrophages, but it is absent from macrophages in T-cell areas of lymph nodes and spleen; low-level expression is detected on blood monocytes.
• Key post-translational modifications include glycosylation and disulfide bond formation, which are critical for the structural integrity of the EGF-like domains.
• Functionally, it is linked to adaptive immunity and calcium-mediated signaling pathways, although its endogenous ligand remains unidentified.

Experimental Guidance and Technical Tips

• For Western blotting (WB), consider a starting dilution in the range of 1:500–1:2000 and validate with a positive control such as mouse macrophage lysates; the predicted molecular weight is ~102 kDa.
• In immunohistochemistry (IHC-P), optimize antigen retrieval conditions and confirm staining specificity in macrophage-rich tissues like spleen or liver; cross-reactivity with rat should be independently verified.
• For paraffin-embedded immunofluorescence (IF-P), use mild fixation and permeabilization protocols to preserve the membrane localization of F4/80.
• This recombinant protein-derived monoclonal antibody may detect both surface and intracellular epitopes; therefore, validate its performance in the specific sample system under study.
• In ELISA applications, checkerboard titrations are recommended when pairing with a detection antibody to identify the optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material sourcing partner dedicated to supporting diagnostic manufacturers, reference laboratories, and research institutes from concept to clinic. For macrophage and immune cell research, we provide a comprehensive portfolio of validated antibody pairs, optimized monoclonal antibodies like F4/80, and bulk ancillary reagents. Our global sourcing capabilities also extend to rare targets and custom configurations, helping you secure the raw materials needed for robust assay development.