Anti-F2RL3 Polyclonal Antibody for WB, ELISA - Q96RI0

Core Product Specifications and Parameters

Biological Background: Proteinase-activated receptor 4 (F2RL3/PAR4) Function and Localization

• Protein Identity: F2RL3, also known as Proteinase-activated receptor 4 (PAR4), Coagulation factor II receptor-like 3, and Thrombin receptor-like 3, is a G protein-coupled receptor that functions as a receptor for activated thrombin or trypsin.
• Signaling and Function: The receptor stimulates phosphoinositide hydrolysis and may play a role in platelet activation.
  Related references: PMID:10079109
• Subcellular Localization: F2RL3 is localized to the cell membrane.
• Tissue Specificity: The gene is widely expressed, with highest levels in lung, pancreas, thyroid, testis, and small intestine; it is not expressed in brain, kidney, spinal cord, or peripheral blood leukocytes. It is also detected in platelets.
• Structural Features: The protein is a single-pass type I membrane protein with a signal peptide, disulfide bonds, glycosylation sites, and seven transmembrane helices, characteristic of the G protein-coupled receptor family.
• Molecular Weight: The calculated molecular weight of F2RL3 is approximately 41 kDa (UniProt: 41133 Da).
• Biological Processes: Keywords associated with F2RL3 include blood coagulation, hemostasis, receptor, transducer, and transmembrane, reflecting its role in coagulation signaling.

Experimental Guidance and Technical Tips

• Immunogen Design: The antibody was generated against a recombinant fusion protein encoding amino acids 20–150 of the N-terminal extracellular domain. This region is critical for receptor activation, so the antibody may recognize both full-length and proteolytically cleaved receptor forms. Consider this when designing assays.
• Western Blot (WB): For WB, expect a band near 41 kDa in human, mouse, and rat samples. Tissues with highest expression (e.g., lung, pancreas) can serve as positive controls, while brain and kidney may be suitable negative controls based on reported tissue specificity.
• ELISA: The antibody is applicable for ELISA; for sandwich ELISA, pairing with a capture or detection antibody targeting a different epitope is recommended. Validate the assay performance using relevant recombinant protein or cell lysates.
• General Recommendations: Optimize experimental conditions (e.g., blocking, antibody dilution) for your specific sample type. Post-translational modifications and sample preparation methods may influence detection, so validate in your system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and biotech companies with a comprehensive one-stop platform for IVD raw material sourcing. Our portfolio includes validated polyclonal and monoclonal antibodies, antibody pairs, and ancillary reagents for targets in thrombosis, hemostasis, and cardiovascular research. If you require bulk quantities, customized formats, or assistance sourcing additional rare targets like F2RL3, our team is ready to support your project from concept to clinic.