Anti-F13A1 Polyclonal Antibody for WB, IF-P, ELISA - P00488

Core Product Specifications and Parameters

Biological Background: F13A1 Function and Localization

• Coagulation factor XIII A chain is the catalytic subunit of the factor XIII fibrin-stabilizing factor complex, which plays a critical role in the final step of the blood coagulation cascade.
• Upon activation by thrombin and calcium ions, factor XIII A chain becomes a transglutaminase that catalyzes the formation of covalent gamma-glutamyl-epsilon-lysine cross-links between fibrin monomers, thereby stabilizing the fibrin clot.
• Additionally, it cross-links alpha-2-plasmin inhibitor and fibronectin to the alpha chains of fibrin, enhancing clot resistance to proteolytic degradation and facilitating wound healing.
• The protein is synthesized as a zymogen and is present both in the cytoplasm of platelets and megakaryocytes, and secreted into the blood plasma, where it circulates as a heterotetramer with two B chains.
• Subcellular localization includes cytoplasm and secreted extracellular space, consistent with its dual intracellular and extracellular roles in hemostasis.
• F13A1 is annotated under keywords such as blood coagulation, hemostasis, metal-binding, and calcium, and is recognized as a disease variant associated with hereditary factor XIII deficiency.
• Post-translational modifications include acetylation and glycosylation, which may influence its stability and secretion.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein corresponding to the C-terminal region (aa 600-732) of human F13A1. This region is distal to the catalytic core and may recognize both zymogen and activated forms. Validate reactivity in your specific assay context.
• For Western blot (WB): The expected molecular weight of full-length human F13A1 is approximately 83 kDa. In reducing conditions, a band near this size is anticipated. Note that in plasma, factor XIII circulates as an A2B2 heterotetramer (~320 kDa), so under non-reducing conditions, higher molecular weight complexes may be observed.
• For immunofluorescence (IF-P): Since F13A1 is both cytoplasmic and secreted, consider permeabilization steps to detect intracellular staining. Fixation conditions should be optimized.
• For ELISA: This polyclonal antibody can be used as either capture or detection reagent. Pairing with a monoclonal antibody may improve sandwich ELISA specificity. Titration and buffer optimization are recommended.
• Cross-reactivity with mouse and rat is reported, but verify in your experimental models to ensure consistent performance.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we support diagnostic manufacturers and research laboratories with reliable one-stop sourcing of IVD raw materials. This anti-F13A1 polyclonal antibody is part of our extensive portfolio for coagulation and hemostasis research, available alongside optimized monoclonal/polyclonal antibodies, validated antibody pairs, and bulk ancillary reagents. Whether you need standard products or custom rare-target sourcing, CamelBio bridges your needs from concept to clinic with efficient supply and technical support.